从虫体提纯HBsAg基因表达产物的新方法  

A Novel Procedure for Purification of HBsAg Produced by a Baculovirus Vector in Insect Larvae

作　　者：邓日强[1] 龙綮新[1] 王珣章[1] 温小昭[1] 蒲蛰龙[1] 

机构地区：[1]中山大学昆虫学研究所,中山大学生物防治国家重点实验室

出　　处：《中山大学学报（自然科学版）》1994年第3期86-90,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

摘　　要：感染含乙型肝炎病毒表面抗原（ＨＢｓＡｇ）基因的粉纹夜蛾重组核型多角体病毒ＴｎＮＰＶ－Ｈｈｓ８５－ＯＣＣ￣＋的粉纹夜蛾幼虫先经匀浆澄清处理后，再用单克隆抗体亲和柱层析的方法提纯ＨＢｓＡｇ蛋白．提纯的蛋白经ＳＤＳ－ＰＡＧＥ，出现３条电泳带，分子量分别为２４ＫＤ，２７ＫＤ和４５ＫＤ．用此法从虫体提纯ＨａｓＡｇ基因表达产物，具有简单、快速、高效的特点．A simple. rapid.and efficient procedure was developed to isolate HBsAgproduced in insect larvae infected with a recombinant TnNPV containing HBsAg gene. The treated homogenates were loaded onto an affinity column. The eluted proteins weredetermined to be 24 kilodaltons(KDa).27KDa and 45KDa. About 200 μg of HBsAgwas obtained from 40g infected larvae.This procedure may be of general utility as a firststep in purification of other proteins synthesized by the recombinant baculoviruses ininsect larvae.

关 键 词：粉纹夜蛾 提纯 基因工程 HBsAg基因 

分 类 号：Q781[生物学—分子生物学]