Biodegradation of azo dyes by genetically engineered azoreductase  

作　　者：WANGJing YANBin ZHOUJi-ti BAOYong-ming LUHong YUANXiao-dong 

机构地区：[1]SchoolofEnvironmentalandBiologicalScienceandTechnology,DalianUniversityofTechnology,Dalian116023

出　　处：《Journal of Environmental Sciences》2005年第4期545-550,共6页环境科学学报（英文版）

基　　金：TheFoundationoftheCross centuryTalentofMinistryofEducationChina

摘　　要：A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.A azoreductase gene with 537 bp was obtained by PCR amplification from Rhodobacter sphaeroides AS1 1737 The enzyme, with a molecular weight of 18 7 kD, was efficiently expressed in Escherichia coli and its biodegradation characteristics for azo dyes were investigated. Furthermore, the reaction kinetics and mechanism of azo dyes catalyzed by the genetically engineered azoreductase were studied in detail. The presence of a hydrazo-intermediate was identified, which provided a convincing evidence for the assumption that azo dyes were degraded via an incomplete reduction stage.

关 键 词：genetically engineered microorganisms AZOREDUCTASE BIODEGRADATION azo dyes 

分 类 号：X70[环境科学与工程—环境工程] Q781[生物学—分子生物学]