一组新发现的肿瘤功能性蛋白质组的指纹图谱  

A newly discovered fingerprint of functional proteome

作　　者：裴毅[1] 郭素堂[2] 王清馨[1] 米振国[2] 赵嘉训[2] 刘维兰[2] 王蓉[3] 韩琳[3] 马小军[3] 古晓东[3] 姚壁[1] 纪丕军[1] 杨永明[1] 魏淑青[1] 

机构地区：[1]山西省肿瘤医院特诊科,太原030013 [2]山西省肿瘤研究所,太原030013 [3]山西医科大学,太原030001

出　　处：《肿瘤研究与临床》2005年第3期153-155,共3页Cancer Research and Clinic

摘　　要：目的确定新发现蛋白质组的指纹图谱及诊断平台。方法将2004年4月至2004年12月31日治疗的146名肿瘤患者用美国赛弗吉(Ciphergen)公司的蛋白质指纹仪及WCX2蛋白质芯片经SELDI检测技术(surfaceenhancedlaserdesorption/ionization-timeofflight-massspectrometer)检测其血清。以质荷比(M/Z)大小构成某一患者血清的蛋白质指纹图谱。结果对272次结果汇总,在M/Z为11100+H～11900+H之间出现质谱峰,其中一个丰度≥20%,其余丰度均≥5%,其峰簇上下游近1000个质量单位内无任何质谱峰,个别情况下,在M/Z为11100+H至11900+H之间出现丰度值≥5%单峰(自命名为LGT蛋白质组lostgoodwilltarget)。结论LGT蛋白质组指纹图阳性诊断平台,在用WCX2蛋白芯片和SELDI技术获取的蛋白质指纹图上,在M/Z为11100+H～11900+H之间出现一质谱峰簇(cluster),其最大丰度≥20%,最小丰度≥5%,并其上游、下游近1000个质量单位内无任何质谱峰出现;在M/Z为11100+H至11900+H之间出现丰度≥5%的单峰指纹标记,其余无变化,视为LGT蛋白质组指纹疑似阳性;在M/Z为11000+H至11900+H之间无任何质谱峰簇,为LGT蛋白质指纹阴性。Objective To identify a newly discovered proteomic fingerprint and its diagnostic application. Methods Serum were collected from 146 cancer patients who were treated in our study group from April to December, 2004. Serum were added to a weak cation exchange 2(WCX2) protein chip, and then analysed on the protein biology system surface enhanced laser desorption ionization time of flight mass spectrometer (SELDI- TOF- MS) manufactured by Ciphergen Biosystems Inc. (Fermont, CA, USA). The proteome fingerprint is shown by M/Z values. Results By summarizing 272 spectra, we discovered a specific mass cluster in the range of M/Z values 11 100+H ～11 900+H, with cluster peaks of amplitudes ≥5 % and one or more than one consecutive peak(s) ≥ 20 % in it. No peak within M/Z values 1 000+H of the upper and lower limits. In particular condition, no cluster appears but a small single peak with amplitude ≥ 5 %-LGT(lost goodwill target). Conclusions On the proteomic fingerprint pattern obtained by using the WCX2 chip SELDI technology, we established the diagnosis criterion as following characteristics according to the existence or amplitude of the proteomic cluster in the range of M/Z values 11 100+H ～ 11 900+H:(1)LGT(+): having a mass cluster, the maximum amplitude ≥20 %, the minimum ≥5 % and no peak within M/Z values 1 000+H of the upper and lower limits. (2)LGT(+/-): having a single peak ≥5 % and no cluster.(3)LGT (-): having no cluster and peak.

关 键 词：恶性肿瘤 蛋白质组 SELDI技术 

分 类 号：R730.4[医药卫生—肿瘤]