LGT蛋白质组对肿瘤患者临床意义的首次观察  

The first observation of the clinical significance of LGT proteome for cancer patients

作　　者：裴毅[1] 郭素堂[2] 王清馨[1] 米振国[2] 赵嘉训[2] 刘维兰[2] 王蓉[3] 韩琳[3] 马小军[3] 古晓东[3] 姚壁[1] 纪丕军[1] 杨永明[1] 魏淑青[1] 

机构地区：[1]山西省肿瘤医院特诊科,太原030013 [2]山西省肿瘤研究所,太原030013 [3]山西医科大学,太原030001

出　　处：《肿瘤研究与临床》2005年第3期156-158,共3页Cancer Research and Clinic

摘　　要：目的初步分析LGT(lostgoodwilltarget)蛋白质组基本临床意义指向。方法将2004年4月至2004年12月31日治疗的146例肿瘤患者用美国赛弗吉(Ciphergen)公司的蛋白质指纹仪及WCX2蛋白质芯片经SELDI检测技术(Surfaceenhancedlaserdesorption/ionization-timeofflight-massspectrometer)检测其血清。以在指纹图上,质荷比(M/Z)为11100+H至11900+H之间出现一峰簇样(cluster)的指纹标志为阳性诊断标准,结合检测次数,共分为6组,观察6组中的死亡率(观察工作于2005年3月20日结束)。结果LGT蛋白质组持续阳性组,死亡率100%;LGT蛋白质组持续阴性组,死亡率为0;LGT蛋白质组由阳性治疗后转成阴性组死亡率为0;LGT蛋白质疑似阳性组,死亡率9.5%;LGT蛋白质组仅查一次阳性组,死亡率17%;LGT蛋白质组查一次阴性组,死亡率1.5%。结论LGT蛋白质组持续阳性,会危及肿瘤患者的生命,由于它的出现距死亡发生有一定时间,所以它是一个早期发现肿瘤恶化、预测死亡发生的早期预警蛋白质组。它的持续阴性表达可以提示肿瘤患者病情稳定,近期没有恶化和死亡的发生,而其从阳性经治疗转阴也可达到此目的。因此,将其作为一种临床诊断平台,以预测肿瘤的发展,并提示相应治疗,对患者长期生存有明确的临床意义。Objective To analyze the clinical significance of LGT(lost goodwill target) proteome. Methods Serum samples were collected from 146 cancer patients who were treated in our studying group from April to December, 2004. Serum samples were added to a weak cation exchange 2 (WCX2) protein chip and analysed on the Protein Biology System surface enhanced laser desorption ionisation time of flight mass spectrometer (SELDI- TOF- MS) manufactured by Ciphergen Biosystems Inc.(Fermont, CA, USA). We repeatedly detected more than 2 times to aggravating patients. We establish the positive diagnosis criterion as following characteristics: having a mass cluster in the range of M/Z values 11,100+H～119,000+H.Being combined with the test times, the cases were divided into 6 groups to investigate the individual mortality. Results Mortality of the six following groups: (1) LGT continuous positive, 100 % (18/18). (2) LGT continuous negative, 0 % (0/16). (3) LGT(+) →(-) after therapy 0 %(0/12). (4) LGT suspected positive, 9.5 % (2/21). (5) LGT positive (only one check), 17% (2/12). (6) LGT negative (one check only) 1.5 % (1/65). Conclusions If LGT proteome was continuously positive, it implies that the disease advanced and predicts a worst case. If LGT proteome was continuously negative, the prognosis is contrary, it means the disease lies stable. Taken it as a diagnosis platform to predict tumor progressing and conducted corresponding treatment, it helps to prolong the survival time of patients.

关 键 词：恶性肿瘤 蛋白质组 SELDI技术 

分 类 号：R730.4[医药卫生—肿瘤]