PCR技术在B细胞淋巴瘤诊断中的应用  

DETECTING B CELL LYMPHOMA BY USING POLYMERASE CHAIN REACTION

作　　者：孙晓非[1] 管忠震[1] 何友兼[1] 崔景华 廖海[1] 陈火胜 

机构地区：[1]中山医科大学肿瘤医院内科

出　　处：《中山医科大学学报》1994年第4期253-255,共3页Academic Journal of Sun Yat-sen University of Medical Sciences

基　　金：卫生部青年科研基金

摘　　要：应用聚合酶链反应（ＰＣＲ）技术从ＤＮＡ水平测定Ｂ细胞非霍奇金淋巴瘤的单克隆起源，以用于淋巴瘤的诊断。４０例标本检测的阳性结果为；Ｂ细胞淋巴瘤１３／１５例，Ｔ细胞淋巴瘤０／３例，霍奇金病０／３例，慢性淋巴结炎０／８例，非淋巴源性肿瘤０／１１。与组织学及免疫组化诊断符合率为８７％。本研究在把分子生物学的新方法用于Ｂ细胞淋巴瘤的临床诊断方面作出初步的探索。onoclonality of B cell lymphoma at DNA level was detected by using the polymerase chain reaction (PCR). Of 40 clinical lymph node biopsy and aspiration specimens obtained from different kinds of diseases , the positive rates of detection were : 13/15 (87%) for B cell lymphoma , 0/3 for T cell lymphoma , 0/3 for Hodgkin's disease , 0/8 for reactive lym- phadenopathy and 0/11 for non-lymphoid tumor. The result above is consistent roughly with that from histology and immunohistochemistry examination. Our study shows that PCR technique is useful for clinical diagnosis of B cell lymphoma.

关 键 词：聚合酶链反应 B细胞淋巴瘤 单克隆 诊断 

分 类 号：R730.43[医药卫生—肿瘤]