正畸牙齿移动过程中大鼠三叉神经节PPTA mRNA表达变化  

Changes in the Expression of PPTA mRNA in the Rat trigeminal Ganglion during and after Tooth Movement.

作　　者：孙应明[1] 罗颂椒[2] 赵昱辉[1] 

机构地区：[1]第四军医大学口腔医学院正畸科,陕西西安710032 [2]四川大学华西口腔医学院正畸科

出　　处：《口腔医学研究》2005年第3期240-242,共3页Journal of Oral Science Research

基　　金：国家自然科学基金资助项目(编号:30200320)

摘　　要：目的:观察正畸牙齿移动不同时期大鼠三叉神经节PPTA mRNA表达变化.方法:采用反转录-聚合酶链反应(RT-PCR)技术检测正畸加力和撤力后不同时期大鼠三叉神经节PPTA mRNA水平的变化情况.结果:加力后2 h大鼠三叉神经节PPTA mRNA的表达量开始增加,加力8 h至加力3 d达到最高,至撤力后2～4周PPTA mRNA的表达量开始下降,但 PPTA mRNA水平仍然明显高于对照组.结论:正畸牙齿移动过程中大鼠三叉神经节PPTA mRNA表达发生了明显的变化.提示P物质可能不仅参与早期正畸牙周组织的炎症损伤过程,而且可能参与了后期的组织修复重建过程.Objectives: To investigate the relationship between substance P and orthodontic tooth movement. Methods: The first maxillary molar was moved mesially by an orthodontic force for 2 hours,8 hours,1 days,3 days,1 week and 2 weeks,whereas the other groups were observed after removal of appliances for 1 week,2 weeks and 4 weeks.The expression of PPTA mRNA in trigeminal ganglion were detected with reverse transcription-polymerase chain reaction(RT-PCR). Results: The expression of PPTA mRNA in trigeminal ganglion was stonger than that in control group at all the time points; PPTA mRNA level was increased after 2 hours and reached the peak level around 8 hours-3 days during tooth movement,4 weeks after tooth movement PPTA mRNA level was still higher than that in the controls. Conclusions: The results suggested the involvement of substance P in both early stages of periodontal remodeling and later in the regenerative processes of the periodontal ligament during and after orthodontic tooth movement.

关 键 词：P物质前速激肽原A(PPTA) 牙齿移动 三叉神经节 反转录-聚合酶链反应 

分 类 号：R783.5[医药卫生—口腔医学]