大鼠骨髓细胞PPARγ和Cbf α1mRNA表达的增龄变化及相关性研究  

Expression of PPAR γ and Cbf α 1 mRNA in bone marrow cells of rat and their correlation with aging

作　　者：丰盛梅[1] 金慰芳[1] 高建军[1] 顾淑珠[1] 

机构地区：[1]复旦大学放射医学研究所骨代谢研究室,上海200032

出　　处：《中国骨质疏松杂志》2005年第2期164-166,183,共4页Chinese Journal of Osteoporosis

摘　　要：目的观察大鼠骨髓细胞中过氧化物酶体增殖物激活受体(peroxisome proliferator-activated receptor γ,PPARγ)与核结合因子α1(cote binding factoralpha l,Cbfα1)表达的增龄性变化,分析二者变化的相关性,探讨老年性骨衰退发生的可能分子机制。方法取1、3、7、12、16、18和 20月龄的SD大鼠,每组雌雄各5只,无菌获得腰椎骨髓细胞,用RT-PCR方法检测骨髓细胞中 PPARγ与Cbfα 1mRNA的表达水平;并采用SPSS11．0统计软件进行差异单因素方差分析和相关分析。结果 PPARγ mRNA的表达水平在3月龄前变化不大,7月龄时下降,约为3月龄的34％ (P<0．01),之后逐渐上调,18月龄约为7月龄的8．8倍(P<0．001);Cbfα1 mRNA表达水平在3 月龄为最高,为1月龄的3倍(P<0．001),随后逐渐下调, 18月龄为最低,约为3月龄的6％ (P<0．001)．PPARγ与Cbfα 1mRNA的表达水平从3月龄开始呈负相关(r=0．5967,P<0．01), 相关系数随月龄增加而增加,16～20月龄期间二者变化的相关性最大。结论 PPARγ mRNA在老年大鼠骨髓细胞中高表达,且与Cbfα 1mRNA的表达呈负相关,提示骨髓细胞PPARγ高表达可能参与老年性成骨细胞前体减少和骨衰退的过程。Objective To investigate the change of peroxisome proliferator-activated receptor γ (PPARγ) and core binding factor alpha 1 (Cbfα 1) genes expression and their correlation in bone marrow cells (BMCs) with aging and the possible molecular mechanism in senile bone diminution. Methods Totally 70 SD rats were divided into 7 different age groups: 1, 3, 7, 12, 16, 18 and 20 month-old group. Each group contained 5 made and 5 female rats. The BMCs were collected from lumbar vertebra (L1-4) . The expression of PPARγ and Cbfα 1 mRNA in BMCs were analysed by semi-quantity RT-PCR. Results The mRNA expression of PPAR γ was stable before 3 months old. But it decreased to 34% of the expression of 3 months old at 7 months (P<0. 01) and reached the highest level at 18 months old (8. 8 times of that of 7 months, P< 0. 001) . The expression of Cbfα 1 mRNA showed the peak value at 3 months old which was 3 times of that of 1 month (P<0. 001) and down-regulated after 3months and achieved the lowest level at 18 months old (6% of that of 3 months old, P<0. 001) . The PPARγ mRNA level showed a negative correlation to Cbfα 1 after 3 months old (r=-0. 5967, P<0. 01 ) . The correlation became stronger with aging and had the strongest relationship during 16-20 menths old (r=-0. 93, F<0. 01 ) during the period of 16 to 20 months old. Conclusion The reduction of osteoblastic progenitors and bone diminution in old population maybe partly due to high expression of in PPARγ BMCs.

关 键 词：PPARΓ mRNA表达 骨髓细胞 CBF 相关性研究 过氧化物酶体增殖物激活受体 增龄变化 receptor RT-PCR方法 单因素方差分析 1mRNA 增龄性变化 分子机制 SD大鼠 相关分析 统计软件 相关系数 老年大鼠 细胞前体 老年性 负相关 

分 类 号：R589[医药卫生—内分泌] R734.2[医药卫生—内科学]