共刺激分子在Behcet病患者结节红斑组织中的表达  

Expression of Costimulatory Molecules in Erythema Nodusum of Patients with Behcet Disease

作　　者：王红[1] 杨培增[2] 彭晓燕[1] 周红颜[2] 黄祥坤[2] 刘云霞[1] 

机构地区：[1]首都医科大学附属北京同仁医院眼科 [2]中山大学中山眼科中心

出　　处：《首都医科大学学报》2005年第3期258-262,共5页Journal of Capital Medical University

基　　金：国家自然科学基金(30400490);北京市科技新星计划(A)类(2003A23);首都医科大学基础临床合作研究基金;北京同仁医院科研基金资助项目

摘　　要：目的探讨Behcet病患者结节红斑中B7:CD28/CTLA-4的表达及意义。方法采用免疫组化方法对5例Behcet病活动期患者腿或臂部结节红斑中CD4、CD8、CD19、CD68、HLA-DR、B7-1、B7-2、CD28和CTLA-4分子表达的情况进行了检测,并用双重染色方法探讨了细胞来源。结果Behcet病结节红斑中表皮约90%的细胞表达B7-1和B7-2,但无CD28和CTLA-4表达。真皮和皮下表达CD4、CD8、CD19、CD68、HLA-DR、CD1a、CD28、CTLA-4、B7-1和B7-2的细胞均显著增多。双染结果显示,100%CD4+T细胞表达CD28分子,47.9%的CD4+T细胞表达CTLA-4分子。100%CD68+细胞表达B7-1和B7-2分子。分别有74.5%和60.8%的HLA-DR+细胞表达B7-1和B7-2分子。结论CD28和CTLA-4分子主要表达于CD4+T细胞,B7-1和B7-2分子主要表达于单核巨噬细胞、抗原提呈细胞和角朊细胞。B7:CD28/CTLA-4通路可诱导巨噬细胞和T淋巴细胞的功能和活性,对Behcet病患者结节红斑的发生、炎症的持续和扩大起重要作用。Objective To investigate the expression and the role of costimulatory molecules B7:CD28/CTLA-4 in erythema nodusum of patients with Behcet disease. Methods Erythema nodusum from 5 patients with progressing Behcet disease and normal skin from 2 healthy individuals were analyzed by immunohistochemical examination for expression of CD4, CD8, CD19, CD68, HLA-DR, B7-1, B7-2, CD28 and CTLA-4 molecules. Labelled cells were visualised using either single or double staining techniques. Results Approximately 90% of epithelial cells in erythema nodusum expressed B7-1 and B7-2 molecules, but no expression of CD28 and CTLA-4. CD4^+Tcells, CD8^+T cells, CD19^+cells, CD68^+cells, HLA-DR^+cells, B7-1^+cells, B7-2^+cells, CD28^+cells and CTLA-4^+cells were increased significantly in the dermis and subcutaneous tissue. Double staining showed that CD28 molecules were expressed on 100% of CD4^+T cells, CTLA-4 molecules were expressed on 47.9% of CD4^+T cells, respectively. All CD68^+ cells expressed B7-1 and B7-2 molecules. B7-1 and B7-2 molecules were expressed on 74.5% and 60.8% of HLA-DR^+cells, respectively. Conclusion CD28 and CTLA-4 molecules are mainly expressed on CD4^+T cells, B7-1 and B7-2 are mainly expressed on macrophages, antigen-presenting cells and keratinocytes. B7:CD28/CTLA-4 costimulatory pathway can induce the function and activation of macrophages and T lymphocytes, and play an important role for the incidence, diffusion and consistence of inflammation in erythema nodusum.

关 键 词：BEHCET病 结节红斑 B7:CD28/CTLA-4 共刺激分子 患者 CD4^+T细胞 T细胞表达 组织 免疫组化方法 CD28分子 单核巨噬细胞 抗原提呈细胞 CD19 CD68 T淋巴细胞 HLA 分子表达 细胞来源 染色方法 CD1A 角朊细胞 CD8 表达及 

分 类 号：R773[医药卫生—眼科] R758.61[医药卫生—临床医学]