靶向hTERT RNA干涉促进人宫颈癌HeLa细胞凋亡的研究  

Study on apoptosis of human cervical carcinoma HeLa cells with RNA interference targeting hTERT

作　　者：赵英 [1] 任君琳 [2] 张瑞 [1] 马龙洋 [1] 鲍炜 [2] 王成济 [1] 杨安钢 [3] 

机构地区：[1]第四军医大学基础部,生物化学与分子生物学教研室,陕西,西安,710032 [2]第四军医大学基础部,免疫学教研室,陕西,西安,710032 [3]第四军医大学基础部,生物化学与分子生物学教研室,陕西,西安,710032,第四军医大学基础部,免疫学教研室,陕西,西安,710032

出　　处：《细胞与分子免疫学杂志》2005年第4期524-526,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家重点基础研究发展规划(973)资助项目(No.2004CB518805);国家高技术研究发展计划(863)资助项目(No.2004AA217071)

摘　　要：目的:利用RNA干涉技术抑制肿瘤细胞端粒酶表达,探讨其对肿瘤细胞凋亡的诱导作用。方法:将成功构建的针对hTERT的siRNA(smallinterferencingRNA)表达载体转染HeLa细胞,通过透射电镜、免疫印迹和流式细胞术(FCM)等方法检测人宫颈癌HeLa细胞的凋亡情况。结果:构建的siRNA表达载体可以诱导HeLa细胞发生凋亡。结论:针对人端粒酶逆转录酶的siRNA表达载体稳定转染HeLa细胞,可诱导HeLa细胞发生凋亡。AIM: To study the induction of tumor cell apoptosis by RNA interference-mediated inhibition of the expression of telomerase in cancer cells. METHODS: HeLa cells were transfected with the successfully established siRNA(small interfering RNA) expression vectors targeting hTERT (human telomerase reverse transcriptase). By electronic microscopy, Western blot and FCM (flow cytometry) the apoptosis of HeLa cells was tested. RESULTS: The established siRNA expression vectors could induce apoptosis of HeLa cells. CONCLUSION: Transfection of siRNA expression vectors targeting hTERT can induce apoptosis of HeLa cells.

关 键 词：RNA干涉 人端粒酶逆转录酶 凋亡 HELA细胞 

分 类 号：R733.7[医药卫生—肿瘤]