大鼠局灶性脑缺血后血脑屏障葡萄糖转运蛋白1表达的变化  

Expression of GLUT1 in blood-brain barrier following cerebral ischemia

作　　者：李相元[1] 李宪章[1] 张爱梅[1] 

机构地区：[1]济宁医学院附属医院,山东济宁272029

出　　处：《山东医药》2005年第17期10-11,共2页Shandong Medical Journal

基　　金：济宁市科技局鉴定成果[2004]第52号

摘　　要：目的研究局灶性脑缺血后血脑屏障葡萄糖转运蛋白1(GLUT1)表达的变化。方法采用线栓法制作大鼠大脑中动脉闭塞模型,用免疫组化方法观察脑缺血后1小时、3小时、6小时、12小时、1天、2天、3天、7天时血脑屏障GLUT1表达的动态变化。结果脑缺血后3小时血脑屏障GLUT1的表达开始增加,以后逐渐升高,1天时达高峰,2天时下降,7天时与假手术组比较无显著差异。结论脑缺血后血脑屏障GLUT1的表达增加;此对缺血脑组织具有保护作用。Objective To investigate the expression of glucose transporter1(GLUT1) in blood-brain barrier following cerebralischemia in rats.Methods Focal permanent cerebral ischemia models were made by occluding the middle cerebral artery with nylon line in rats.The expression of GLUT1 in blood-brain barrier were investigated by immunohistochemistry staining.Results The expression of GLUT1 in blood-brain barrier started to increase at 3h after cerebral ischemia,then increased gradually,peaked at 24h,decreased at 2d.There was no significant difference at 7d compared with sham operation group.Conclusions The expression of GLUT1 in blood-brain barrier is upregulated following cerebral ischemia.It may have a protective effect on cerebral infarction.

关 键 词：血脑屏障 葡萄糖转运蛋白1 缺血后 局灶性 大鼠 GLUT1 动脉闭塞模型 免疫组化方法 GLUTl 缺血脑组织 动态变化 保护作用 天时 线栓法 

分 类 号：R651.15[医药卫生—外科学] R972[医药卫生—临床医学]