Bio-control trials of Chaetomium spirale ND35 against apple canker  

作　　者：XINYa-fen SHANGJin-jie 

机构地区：[1]ShanghaiVocationalTechnicalCollegeofAgriculture＆Forestry2ZhongshanRoad,Songjiang,Shanghai.201600,P.R.China [2]DongvingForestryBureauDongyingCity25700,ShandongProvince,P.R.China

出　　处：《Journal of Forestry Research》2005年第2期121-124,共4页林业研究（英文版）

基　　金：This research was supported by National Natural Science Foundation of China (Grant No. 30100143)

摘　　要：A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populus tomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa Canker of apple were preliminarily investigated. The results of dual culture on PDA plate showed that C. spirale ND35 was capable of strong antagonism against Valsa ceratosperma, and for inhibiting the mycelial growth of V. ceratosperma,.the crude extract of liquid culture of corn steep powder broth was more effective than that one of malt extract broth (MEB). The results of bio-control in greenhouse and field indicated that the disease incidence of apple tree treated with C. spirale ND35 was lower significantly than that treated by other methods. The re-isolation experiment suggested that C. spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35 colonization rate of the treatment with solid wheat bran culture was higher than that of corn steep powder broth, but the field experiment result the control effect of liquid culture of C. spirale ND35 was better than that of solid culture.A new endophytic antagonistic fungus, Chaetomium spirale ND35 from Populustomentosa, was reported. The bio-control trials of C. spirale ND35 against the Valsa Canker of applewere preliminarily investigated. The results of dual culture on PDA plate showed that C. spiraleND35 was capable of strong antagonism against Valsa ceratosperma, and for inhibiting the mycelialgrowth of V. ceratosperma,, the crude extract of liquid culture of corn steep powder broth was moreeffective than that one of malt extract broth (MEB). The results of bio-control in greenhouse andfield indicated that the disease incidence of apple tree treated with C. spirale ND35 was lowersignificantly than that treated by other methods. The re-isolation experiment suggested that C.spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35colonization rate of the treatment with solid wheat bran culture was higher than that of corn steeppowder broth, but the field experiment result the control effect of liquid culture of C. spiraleND35 was better than that of solid culture.

关 键 词：chaetomium spirale apple tree canker ANTAGONISM BIO-CONTROL valsaceratosperma 

分 类 号：S436.611.1[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]