丹磺酰氯作为生物胺柱前衍生试剂衍生化条件的研究  

Study on derivatising condition of biogenic amines with dansyl chloride as pre-column derivatising agent

作　　者：董伟峰[1] 李宪臻[1] 林维宣[2] 

机构地区：[1]大连轻工业学院生物与食品工程学院,辽宁大连116034 [2]辽宁出入境检验检疫局,辽宁大连116001

出　　处：《大连轻工业学院学报》2005年第2期115-118,共4页Journal of Dalian Institute of Light Industry

摘　　要：报道了荧光衍生试剂丹磺酰氯同多种生物胺进行衍生化反应条件的实验研究。在丹磺酰氯浓度为9mg/mL、反应温度为60℃、反应时间为15min、缓冲体系的pH值为11.5时,衍生效果最佳。衍生试剂和衍生物的稳定性分别可达一个月和半年以上,即可以和伯胺反应又可以和仲胺反应。This paper reports the experimental investigation on the derivatising condition of biogenic amines with dansyl chloride as fluorescent derivation agent. The most suitable derivatising condition are determined as follows: concentration of dansyl chloride, 9?mg/mL; temperature of reaction, 60?℃, time of reaction, 15?min; and pH of buffer, 11.5. The Dns-Cl derivation agent is not decomposed within a month and the dansyl derivative, within six months. The Dns-Cl derivational agent may derivative with primary amine and secondary amine.

关 键 词：丹磺酰氯 柱前衍生试剂 衍生化 生物胺 荧光衍生试剂 实验研究 反应条件 反应温度 反应时间 缓冲体系 氯浓度 PH值 稳定性 衍生物 

分 类 号：O625.752[理学—有机化学] O623.726[理学—化学]