胃癌组织中EphB_2受体胞外区结构域的克隆和序列测定  

Cloning and sequencing of the ectodomain of EphB_2 receptor in the tissue of gastric carcinoma

作　　者：韩景田[1] 陈小义[1] 张晓光[1] 

机构地区：[1]武警医学院中心实验室,天津300162

出　　处：《国外医学（临床生物化学与检验学分册）》2005年第3期133-135,共3页Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine

摘　　要：目的　对胃癌组织中酪氨酸蛋白激酶 EphB2 受体胞外区结构域进行克隆。方法　从胃癌患者手术切片标本中提取RNA,进行RT PCR,将扩增片段克隆入 pUC19质粒,进行测序及酶切鉴定。结果　克隆片段序列正确。结论　为进行表达、筛选与之相互作用的蛋白,阐明其生理功能打下基础。Objective To clone the ectodomain of EphB 2 receptor tyrosine kinase in the tissue of gastric carcinoma.Methods Total RNA was extracted from a surgical specimen of human gastric carcinoma, and primers were designed by computer analysis and chemically synthesized. The amplified fragment was obtained by RT-PCR and cloned into pUC 19 vector. The recombinant clones were confirmed by sequencing and restriction endonuclease cleavages. Results The cloned fragment was identical to the design.Conclusion It will offer the basis for further investigation including identify its new ligand, the expression, and biological functions.

关 键 词：胃癌 癌组织 EphB2受体 克隆 序列测定 聚合酶链反应 

分 类 号：R735.2[医药卫生—肿瘤] R392.12[医药卫生—临床医学]