利尿配方注射液中细菌内毒素定量测定的研究  

Study on bacterial endotoxin in diuretic prescription by kinetic turbidimetric limulus

作　　者：林秋晓[1] 劳海燕[1] 王仁土[1] 

机构地区：[1]广东省人民医院药学部,广东广州510080

出　　处：《中国现代应用药学》2005年第3期234-236,共3页Chinese Journal of Modern Applied Pharmacy

基　　金：广东省重点科技攻关项目;项目编号:2KM054063

摘　　要：目的应用动态比浊法鲎试验定量测定利尿配方注射液的细菌内毒素含量。方法对样品中定量添加标准内毒素进行干扰试验,通过回收率定最佳检测浓度再进行三个批号样品的正式干扰试验,样品中定量添加标准内毒素,其回收率均在为50%～200%内。结果标准内毒素使用5.00,0.500,0.0500Eu/mL,将样品制备成最佳浓度为定量添加标准内毒素,无干扰作用,可用于有效的日常检查。结论动态比浊法鲎试验可以高效地测定利尿配方注射液细菌内毒素含量。OBJECTIVE The kinetic turbidimetric limulus test was used in the bacterial endotoxin assay of diuretic prescription. METHODS Spiking an amount of standard endotoxin into diuretic prescription samples, the interference test was fulfiled to detect the recovery in the range of 50%~200%. RESULTS With standard endotoxion working curve was 5.00、0.500、0.0500EU/mL.A 4-fold dilution to diuretic prescription was effective to eliminate the interference in limulus test. CONCLUSION The kinetic turbidimetric limulus test povides a new way to the detection of endotoxin in diuretic prescription.

关 键 词：动态比浊法鲎试验 酚妥拉明 多巴胺 呋塞米 细菌内毒素 干扰试验 

分 类 号：R927.12[医药卫生—药学]