防治辣椒疫病的芽孢菌株的筛选及其抑菌效果的测定  被引量:20

Screening Test and Inhibiting Effect of Spore Bacteria to Phytophthora capsici

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作  者:严占勇[1] 肖崇刚[1] 易龙[1] 王开真[1] 田艳[1] 

机构地区:[1]西南农业大学植物保护学院

出  处:《中国蔬菜》2005年第6期18-20,共3页China Vegetables

摘  要:从辣椒根际土壤中分离出293个分离物,通过平板对峙培养,筛选出对辣椒疫霉菌有较强拮抗作用的5个芽孢菌株。室内测定其对辣椒疫霉菌的抑菌带宽达8.0-11.3mm。温室控病试验表明,芽孢菌Bn-130对辣椒疫病防治效果最好,达64.7%。无菌滤液试验表明,拮抗菌Bn-130无菌滤液在一定浓度范围内能有效地抑制菌丝生长,减少孢子萌发。293 isolates were obtained from rhizosphere soil of pepper. It was found that 5 potential biocontrol spore bacteria showed good antagonistic action on Phytophthora capsici by pairing culture on CAA. The inhibition zone diameter ranged from 8.0 mm to 11.3 mm. The result from the experiment at greenhouse showed that Bn - 130 isolate could effectively control the pepper blight, and the control efficiency reached 64. 7 %. The culture filtrate of Bn - 130 isolate was effective for inhibiting mycelium growth and reducing spore germination of P. capsici in certain concentration.

关 键 词:辣椒疫病 抑菌效果 筛选 菌株 辣椒疫霉菌 根际土壤 对峙培养 拮抗作用 室内测定 防治效果 菌丝生长 浓度范围 孢子萌发 分离物 芽孢菌 拮抗菌 试验 滤液 无菌 温室 

分 类 号:S436.418[农业科学—农业昆虫与害虫防治]

 

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