致敏树突状细胞介导CTL抗骨髓瘤免疫的实验研究  

Research of Specific Cytotoxicity T Lymphocyte Response on Myeloma Cells Induced by Myeloma Antigen-Pulsed Dendritic Cells

作　　者：邓晓辉[1] 梁辉[1] 陈琳军[1] 

机构地区：[1]上海第二医科大学新华医院血液科,上海200092

出　　处：《上海第二医科大学学报》2005年第5期447-449,482,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市科技发展基金(4119024)资助项目.

摘　　要：目的比较两种树突状细胞(骨髓瘤致敏DC和未致敏DC)诱导的细胞毒T淋巴细胞抗自身骨髓瘤的细胞免疫。方法用多发性骨髓瘤(MM)患者骨髓CD34+细胞诱生DC,将MM患者骨髓瘤冻融物冲击致敏DC。MTT法检测骨髓瘤抗原致敏和未致敏DC诱导的自身T细胞对患者骨髓瘤细胞的杀伤率。结果骨髓瘤冻融物致敏DC诱导的自身T细胞对患者骨髓瘤细胞的杀伤率远大于非致敏DC。结论患者骨髓瘤冻融物致敏的DC能有效诱导自身T细胞抗骨髓瘤免疫。Objective To compare cell immunity of cytotoxic T lymphocyte response on myeloma cells induced by dendritic cells with or without myeloma antigen. Methods Dendritic cells (DC) were cultured by CD34 + cells from bone marrow of multiple myeloma (MM) patients. DCs were pulsed with myeloma lysates from MM patients. The killing rates of autologous T lymphocytes induced by DC pulsed with or without myeloma antigen on myeloma cells were measured by MTT method. Results Autologous T lymphocytes induced by DCs pulsed with myeloma lysates had stronger killing effect on myeloma cells than T lymphocytes induced by non myeloma antigen pulsed DC. Conclusion DCs pulsed with myeloma lysates from MM patients can effectively induce autologous T cells anti-myeloma cell immunity.

关 键 词：树突状细胞介导 实验研究 骨髓CD34^+细胞 CTL 细胞毒T淋巴细胞 骨髓瘤细胞 多发性骨髓瘤 MTT法检测 DC诱导 T细胞 细胞免疫 冲击致敏 抗原致敏 杀伤率 患者 自身 冻融 

分 类 号：R733.3[医药卫生—肿瘤] R259.565[医药卫生—临床医学]