羟基喜树碱联合热疗抑制人肝癌细胞SMMC-7721体外增殖作用的研究  

THE RESEARCH ON THE COMBINATION OF HYDROXYCAMPTOTHECIN AND HYPERTHERMIA AGAINST HEPATOCELLULAR CARCINOMA CELL LINE SMMC-7721 IN VITRO

作　　者：杨阳[1] 刘宝瑞[1] 钱晓萍[1] 

机构地区：[1]南京大学医学院附属鼓楼医院肿瘤中心,江苏南京210008

出　　处：《实用临床医药杂志》2005年第5期5-8,共4页Journal of Clinical Medicine in Practice

基　　金：江苏省卫生厅重点资助项目(H200349)

摘　　要：目的探讨羟基喜树碱联合热疗体外抑制人肝癌细胞增殖的效果,以确定联合方案是否具有协同效应。方法使用MTT法测定单独或联合应用羟基喜树碱与热疗对人肝癌SMMC7721细胞的体外增殖的抑制作用,使用流式细胞仪检测不同方案对SMMC7721细胞凋亡率的影响。结果联合应用热疗可以显著提高羟基喜树碱引起的细胞增殖抑制及细胞凋亡。以亚毒性剂量浓度的羟基喜树碱为例,对照组、化疗组、热疗组、热化疗组的细胞增殖抑制率分别为0%、13.65%、32.46%、71.89%;细胞倍增时间分别为40.54、86.35、106.85、187.90h;细胞凋亡率分别为2.19%、3.96%、10.16%、20.42%。结论联合应用热疗与羟基喜树碱,对SMMC-7721细胞体外增殖的抑制及诱导细胞凋亡具有显著的协同作用。Objectives To investigate whether concurrent exposure of human hepatocellular carcinoma cells SMMC-7721 to hydroxycamptothecine with hyperthermia will increase anticancer effects. Methods The MTT assay has been developed for quantitative evaluation of the proliferation of SMMC-7721 and cytotoxicity of hydroxycamptothecine with or without hyperthermia. Flow cytometric analyses were used for the assessment of apoptosis rates after treatment. Results The combined treatments significantly induced apoptosis and cytotoxicity higher than exposure to 43 ℃ hyperthermia alone or hydroxycamptothecin alone. Conclusion While both hyperthermia and hydroxycamptothecin can individually induce apoptosis and anti-proliferation effect, they may offer synergistic benefits when used concurrently.

关 键 词：热疗 羟基喜树碱 SMMC-7721细胞株 

分 类 号：R730.54[医药卫生—肿瘤] R735.7[医药卫生—临床医学]