塞来昔布对肺癌细胞的增殖抑制作用研究  

THE INHIBITORY EFFECT OF CELECOXIB ON LUNG CANCER CELL LINE IN VITRO

作　　者：郑建[1] 周丹[2] 陈鸿义[2] 刘桐林[2] 李简[2] 

机构地区：[1]武警江苏总队医院胸外科,江苏扬州225003 [2]北京大学第一医院胸外科,北京100034

出　　处：《实用临床医药杂志》2005年第5期46-48,共3页Journal of Clinical Medicine in Practice

摘　　要：目的研究非甾体类抗炎药塞来昔布对肺癌细胞的体外抑制作用。方法选用肺癌A549细胞系体外培养,在不同塞来昔布浓度作用下,MTT法检测肺癌细胞的增殖抑制,流式细胞仪测定肺癌细胞周期分布以及RTPCR法观察肺癌细胞血管内皮生长因子mRNA(VEGFmRNA)的表达水平。结果塞来昔布对肺癌细胞增殖有抑制作用,且呈剂量依赖关系,IC50为50μmol/L;塞来昔布可将肺癌细胞阻滞在G0/G1期,有剂量依赖性;RTPCR法中,对照组肺癌细胞VEGFmRNA的表达水平高于药物组(P<0.05),药物组中肺癌细胞VEGFmRNA表达水平与塞来昔布浓度相关。结论塞来昔布对肺癌细胞有明显的体外增殖抑制作用,与VEGFmRNA的表达下调和阻滞细胞周期在G/G期有关。Objective To observe that celecoxib, a selective inhibitor of cyclooxygenase-2(COX-2), in vitro, inhibited proliferation of cell line of lung cancer. Methods Different doses of celecoxib were added into the lung cancer cell line of A549 to demonstrate its biological function. MTT was performed to reveal the inhibitory effect on cell proliferation. Flow cytometry was used to analyze the effect of celecoxib on cell cycle. VEGF mRNA level in A549 was measured with RT-PCR. Results There was a does-dependent inhibition of cell proliferation by celecoxib. IC50 was 50 μmol/L. Celecoxib induced G_0/G_1 cell cycle arrest, inhibited the G_1 cells to Sphrase transition. Higher level of VEGF mRNA was observed in control than that in test group (P<0.05). In addition, the expression level of VEGFmRNA correlated with the concentration of celecoxib. Conclusion Celecoxib inhibited the proliferation of lung cancer cell in vitro, which may account for its inhibitory effect of angiogenesis and iduction of G_0/G_1 cell cycle arrest.

关 键 词：肺癌 塞来昔布 环氧合酶-2 

分 类 号：R734.2[医药卫生—肿瘤]