The Synergistic Inhibitory Effect of STI571 in Combination with Arsenic Trioxide （As2O3） on Multidrug-Resistant Leukemic Cells  

作　　者：LiChen JianminWang XiaopingXu LeiGao XinhongFei JingweiLou ZhengxiaHuang 

机构地区：[1]DepartmentofHematology,ChanghaiHospitalofTheSecondMilitaryMedicalUniversity,Shanghai200433,China

出　　处：《Chinese Journal of Clinical Oncology》2005年第3期671-674,共4页中国肿瘤临床（英文版）

摘　　要：OBJECTIVE To study the synergistic effect of STI571, an inhibitor of tyrosine kinase, in combination with arsenic trioxide As2O3 on a multidrug-resistant leukemia cell line expressing bcr-abl. METNODS The cytotoxic effect of STI571 alone or in combination with different concentrations of As2O3 on the bcr-abl and mdrl -positive leukemia cell line, K562-n/VCR, was examined by the MTT method. RESULTS One μmol/L of STI571 alone had no significant cytotoxic effect on K562-n/VCR cells. However the cytotoxic effect increased markedly when combined with As2O3 at concentrations of 10^-5, 10^-6, 10^-7 and 10^-8 mol/L. The IC50 of K562-n/VCR cells in As2O3 group was 1.879 μmol/L, with. Upon addition of STI571, the IC50 decreased to 0.155 μmol/L resulting in a synergistic cytotoxic effect on K562-n/VCR cells that was increased 12.1 times. CONCLUSION A combination of STI571 with As2O3 has a more powerful inhibitory effect on leukemia cells expressing positive bcr-abl and positive mdrl compared to the effect with As2O3 alone.

关 键 词：STI571 抑制作用 三氧化砷 白血病 肿瘤细胞 

分 类 号：R733.7[医药卫生—肿瘤]