奥曲肽对胃癌细胞株SGC7901生长调控作用的研究  

Experimental study of regulatory effect of octreotide on human gastric cancer cell line SGC7901 growth in vitro

作　　者：张南征[1] 马晓燕[2] 

机构地区：[1]解放军第97医院消化内科,江苏徐州221004 [2]徐州医学院肿瘤学教研室,江苏徐州221006

出　　处：《东南国防医药》2005年第3期166-168,180,共4页Military Medical Journal of Southeast China

基　　金：军队医药卫生科研基金课题 (0 2 MA0 2 1)

摘　　要：目的　研究奥曲肽(OCT)对人胃中分化腺癌细胞株SGC790 1体外生长抑制作用的机制。方法　MTT法测定OCT对SGC790 1细胞生长的调控;流式细胞术分析OCT对SGC790 1细胞周期的影响;免疫细胞化学染色法检测表皮生长因子受体(EGFR)的表达;放射免疫法测定细胞培养上清液中表皮生长因子(EGF)和胃泌素的含量。结果　OCT对SGC790 1细胞生长有抑制作用;OCT可诱导SGC790 1细胞G0 / G1期阻滞,加药后2 4小时最显著,同时伴S期细胞比例减少;OCT作用后2 4、36小时,SGC790 1细胞EGFR表达较对照组明显减少。加入OCT2 4小时,细胞培养上清液中EGF和胃泌素含量明显下降。结论　OCT可通过多种途径抑制体外培养的SGC790 1细胞的增殖。Objective To study the mechanisms that regulatory effect of somatostatin analogue(octreotide,OCT)on the growth of gastric cancer cell line SGC7901 in vitro.Methods Cell proliferation was evaluated by means of MTT assay; cell cycle distribution was measured by flow cytometric analysis . The immunocytochemical stain and image analysis were applied to measure the expression of EGFR protein.The contents of EGF and gastrin were determined by radioimmunoassay (RIA). Results The growth of human gastric cancer cell line SGC7901 was inhibited by OCT.OCT also induced SGC7901 arrest at G0/G1 phase and S phase decrease, which peaked at 24h. OCT regulated downwards the expression of EGFR. At 24h,the contents of EGF and gastrin were significantly decreased by OCT. Conclusions OCT inhibits the growth of gastric cancer cell line SGC7901 by many pathways in vitro.

关 键 词：奥曲肽 生长抑素 胃肿瘤 细胞周期 

分 类 号：R735.2[医药卫生—肿瘤]