木霉菌原生质体的制备和再生的研究  

The Production and Regeneration of Trichoderma Protoplast

作　　者：刘限[1] 高增贵[2] 庄敬华[2] 肖淑芹[2] 陈捷[2,3] 

机构地区：[1]沈阳农业大学生物科学技术学院 [2]沈阳农业大学植物保护学院 [3]上海交通大学农业与生物学院,上海201101

出　　处：《沈阳农业大学学报》2005年第1期37-40,共4页Journal of Shenyang Agricultural University

基　　金："十五"国家攻关项目(2001BA509B05);辽宁省自然科学基金项目(2001101041)

摘　　要：采用正交拉丁设计对影响木霉菌原生质体制备和再生的条件进行了系统研究。结果表明:木霉菌原生质体制备受到缓冲体系、渗透压稳定剂、木霉菌菌龄、细胞壁降解酶的种类、酶解时间和再生培养基的影响,而不受木霉菌株系的影响。其中以磷酸缓冲体系和蔗糖为渗透压调节剂的降解体系为最佳,木霉菌菌龄以培养20h较好,崩溃酶对木霉菌细胞壁的降解效果好,酶解时间以4h为最佳,再生培养基以基础培养基和NaCl为渗透压调节剂为最佳。The production and regeneration of protoplast of Trichoderma were studied. The results showed that the production and regeneration of protoplast were affected by buffering system, osmotic stabilization agent, hyphae age, cell wall degeneration enzyme, degeneration time and regeneration medium, but not by the different Trichoderma srains. Therefore PBS plus the sucrose as osmotic stabilization agent, hyphae from 20h-culture ,driselase as cell wall degeneration enzyme, 4h enzymolysis as well as basal medium plus osmosatic agent NaCl as major components of regeneration medium was the best reaction conditions for Trichoderma protoplast production and regeneration .

关 键 词：木霉菌 原生质体制备 渗透压调节剂 再生培养基 渗透压稳定剂 细胞壁降解酶 缓冲体系 酶解时间 基础培养基 系统研究 降解效果 NaCl 最佳 菌龄 株系 

分 类 号：S436.461[农业科学—农业昆虫与害虫防治] S646.190.3[农业科学—植物保护]