CD95、Bcl-2和TGF-α表达在慢性肾衰及血液透析患者的临床应用  

Clinical Meanings of Changes of Blood Expression of CD95 Antigen (Fas), Bcl-2 and Transforming Growth Factor-α (TGF-α) in Patients with Chronic Renal Failure on Hemodialysis

作　　者：杨道理[1] 罗南萍[1] 孙晓明[1] 

机构地区：[1]济南军区总医院实验诊断科,250031

出　　处：《放射免疫学杂志》2005年第3期161-163,共3页Journal of Radioimmanology

摘　　要：目的:分析慢性肾功能衰竭患者及血液透析前后CD95抗原(Fas)、抗细胞凋亡因子(Bcl-2)蛋白及转化生长因子-α(TGF-α)含量变化及相互关系。方法:采用流式细胞术及放射免疫分析,分别测定患者血液透析(HD)前后外周血单个核细胞中CD95、Bcl-2阳性细胞数及血清转化生长因子-α的表达。结果:透析组、非透析组CD95明显高于对照组。Bcl-2与TGF-α含量均明显低于对照组(P<0.01)。HD后CD95表达明显高于HD前(P<0.05),Bcl-2蛋白在HD前后无显著性差异(P>0.05)。结论:CD95、Bcl-2及TGF-α表达在慢性肾功能衰竭及血液透析过程中起重要作用。Objective To study the changes of expression of blood CD95 antigen (Fas), and anti-apoptosis factor (Bcl-2) and TGF-α after hemodialysis in patients with chronic renal failure. Methods The percentage of CD95 and Bcl-2 positive cells in peripheral blood monocytes were examined with flow cytometry and serum TGF-α contents were measured with RIA in 40 patients with chronic renal failure both before and after hemodialysis as well as in 25 other patients with chronic renal failure but not on dialysis and 30 controls. Results Expressions of CD95 were significantly higher and expressions of Bcl-2, TGF-α were significantly lower in all the patients with chronic renal failure than those in the controls (P<0.01). In patients on dialysis, the CD95 expressions increased significantly after dialysis (vs before dialysis, P<0.05), but Bcl-2 expressions were not significantly changed (P>0.05). Conclusion The up-regutation of CD95 expression and increase of serum TGF-α contents after hemodialysis might contribute to induction of apoptosis of mesangial cells, which would be beneficial to the patient.

关 键 词：TGF-Α 血液透析患者 临床应用 慢性肾衰 血清转化生长因子-α 慢性肾功能衰竭患者 外周血单个核细胞 抗细胞凋亡因子 Bcl-2蛋白 CD95抗原 放射免疫分析 流式细胞术 显著性差异 透析前后 相互关系 含量变化 透析过程 对照组 

分 类 号：R735.7[医药卫生—肿瘤] R459.5[医药卫生—临床医学]