小檗碱改善高脂饮食大鼠的胰岛素抵抗  

Berberine Improves Insulin Resistance Induced by High Fat Diet in Rats

作　　者：周丽斌[1] 杨颖[1] 尚文斌[1] 李凤英[1] 唐金凤[1] 王晓[1] 刘尚 袁国跃[1] 陈名道[2] 

机构地区：[1]上海第二医科大学附属瑞金医院上海市内分泌代谢病研究所上海市内分泌代谢病临床医学中心 [2]上海第二医科大学附属瑞金医院上海市内分泌代谢病研究所上海市内分泌代谢病临床医学中心全

出　　处：《放射免疫学杂志》2005年第3期198-200,共3页Journal of Radioimmanology

基　　金：上海联合利华研究与发展基金资助项目(2001)

摘　　要：目的:观察小檗碱是否能改善高脂饮食诱导的胰岛素抵抗,以探讨小檗碱干预糖耐量受损(IGT)的可能性。方法:8周龄雄性SD大鼠29只,分为正常组(NC,n=9)和高脂组(HF,n=20)。高脂饲料喂养14周后高脂组分为二组,10只大鼠继续喂养高脂饮食,另一小檗碱组(HF+B,n=10)每天灌胃小檗碱150mg/kg体重,治疗6周后进行口服葡萄糖耐量试验和胰岛素耐量试验(ITT),评估小檗碱对胰岛素敏感性的影响。结果:HF组大鼠体重、肝重和附睾脂肪重量均明显高于HF+B和NC组(均P<0.01),HF+B组空腹血糖和葡萄糖负荷后2h血糖明显低于HF组(分别为5.70±0.52mmol/Lvs6.66±0.51mmol/L和7.88±0.46mmol/Lvs8.85±1.01mmol/L),空腹和葡萄糖负荷后2h胰岛素HF+B组也显著低于HF组(分别为0.63±0.25ng/mlvs1.64±0.68ng/ml和1.20±0.21ng/mlvs3.60±0.36ng/ml)。各时间点血糖和胰岛素HF组均显著高于NC组(均P<0.01)。Homa胰岛素抵抗指数HF组明显高于HF+B组(P<0.01)。ITT腹腔注射胰岛素后各时间点血糖下降幅度HF+B组均高于HF组,15min时HF+B组血糖下降23%,而HF组仅下降7%。结论:长期高脂饮食可导致大鼠胰岛素抵抗,小檗碱明显降低高脂大鼠的高胰岛素血症,改善胰岛素抵抗,因此适合于IGT的干预。Objective To observe the effect of berberine on insulin resistance induced by high fat diet in rats. Methods Normal male SD rats (8 weeks old) were divided into two groups taking either normal chow (NC, n=9) or high fat diet (HF, n=20). After fourteen weeks, HF rats were divided into two groups. Ten rats continued to take high fat diet. Another ten rats took additional berberine gavage (HF+B,150mg/kg weight once a day). Six weeks later, oral glucose tolerance test and insulin tolerance test were performed for estimating insulin sensitivity. Results The body weight, liver weight and epididyaml fat pads weight of HF group were significantly higher than those of HF+B group and NC group (all P<0.01). Fasting plasma glucose, insulin and plasma glucose, insulin 2h after taking glucose in HF+B rats were significantly lower than those in HF rats (all P<0.01). Plasma glucose and insulin levels at all time points in HF rats were significantly higher than those in NC rats. Homa-IR of HF group was markedly higher than that of HF+B group (P<0.01). The glucose-lowering effects after the administration of insuin (0.5u/kg intrapenitoneally) at all time points in HF+B rats were stronger than those in HF rats with 23% and 7% reduction at 15min respectively. Conclusion Long term high fat diet resulted in insulin resistance. Berberine was able to reverse insulin resistance through promoting peripheral tissue uptaking of glucose and decreasing insulin, which would be quite ideal for the intervention of IGT.

关 键 词：高脂饮食 小檗碱 Homa胰岛素抵抗指数 口服葡萄糖耐量试验 胰岛素耐量试验 胰岛素敏感性 高胰岛素血症 糖耐量受损 mol/L 注射胰岛素 饮食诱导 SD大鼠 高脂饲料 大鼠体重 2H血糖 空腹血糖 高脂大鼠 负荷后 HF B组 可能性 

分 类 号：R589.2[医药卫生—内分泌] R284.1[医药卫生—内科学]