关节内注射曲安奈德对山羊颞下颌关节骨关节病的影响  

Histopathological study of the effects of intra-articular injection of triamcinolone acetonide on temporomandibular joint osteoarthrosis in goats

作　　者：袁奎封[1] 来庆国[1] 宋代辉[2] 石荣纪[1] 

机构地区：[1]山东大学第二医院口腔颌面外科,山东济南250033 [2]山东大学第二医院口腔医院口腔颌面外科,山东济南250033

出　　处：《上海口腔医学》2005年第3期277-280,共4页Shanghai Journal of Stomatology

摘　　要：目的:观察关节内注射曲安奈德后,山羊颞下颌关节骨关节病的组织病理学变化。方法:在10只山羊的双侧颞下颌关节上腔一次性注射Ⅰ型胶原酶0.8ml,诱导制作山羊骨关节病模型;随机将动物分为对照组和治疗组,每组5只,分别在注射Ⅰ型胶原酶后30、60d后,进行第1次和第2次治疗;第1次治疗90d后,处死2组动物,切取关节标本,进行光镜和扫描电镜观察,并对2组关节光镜下9个区的组织病理学变化进行评分,每个区得出平均分后,应用SAS统计软件GLM模型对治疗组和对照组的分数进行统计学处理,评价有无差异。结果:光镜和扫描电镜下观察,对照组髁突、关节盘、关节窝表现为骨关节病变化,治疗组表现出较对照组更严重的病理变化。光镜下治疗组与对照组的组织学得分有显著性差异(P<0.01)。结论:关节上腔注射曲安奈德,能加重骨关节病的程度。PURPOSE:The aim of this study was to investigate the histopathological changes of human temporomandibular joint osteoarthrosis following intra-articular injection of triamcinolone acetonide, and compare the results to these of control group. METHODS: The upper compartments of both joints of 10 goats were injected with 0.8 ml collagenase I to induce osteoarthrosis. The 10 goats were then equally divided into control group and experimental group. The first and second treatment were carried out 30 and 60 days after injection of collagenase I, respectively. The animals were sacrificed 90 days after the first treatment. The specimens of the animal TMJ were harvested, histologically examined under light microscopy and scanning electron microscopy, and scored in a subjective manner following the criteria which was a modification of the method of Mankin et al and Yoshimi et al. GLM model in SAS software package was adopted to compare the differences between the control group and experimental group. RESULTS: Under light microscopy and scanning electron microscopy, the condyle, meniscus and glenoid fossa of the control group demonstrated distinct osteoarthrosis; the histopathologic changes in the experimental group was more serious than the control group, and the differences of the histologic scores were significant between the two groups (P<0.01). CONCLUSION: Intra-articular injection of triamcinolone acetonide as a lytic agent will worsen for articular cartilage reparation.

关 键 词：颞下颌关节 骨关节病 曲安奈德 

分 类 号：R782.6[医药卫生—口腔医学]