IL-10 mRNA及其蛋白在慢性牙周炎牙龈组织中的表达  

Study on the expression of IL-10 mRNA and the protein in gingival tissues of patients with chronic periodontitis

作　　者：程培红[1] 戚向敏[2] 孙善珍[2] 刘琳[3] 

机构地区：[1]山东大学齐鲁医院口腔内科 [2]山东大学口腔医院口腔内科,山东济南250012 [3]济南市第五人民医院口腔科,山东济南250022

出　　处：《上海口腔医学》2005年第3期284-288,共5页Shanghai Journal of Stomatology

摘　　要：目的:检测IL-10mRNA及其蛋白在慢性牙周炎牙龈组织中的表达及其组织细胞来源。方法:随机选择12例慢性牙周炎翻瓣术患者作为牙周炎组,10例龈切术患者作为牙龈炎组,6例阻生牙拔除术患者作为健康对照组。分别采用原位杂交和免疫组化检测技术,检测各组牙龈标本中IL-10的表达。每组IL-10mRNA及蛋白两种水平间的比较采用秩和检验;各组间数据的两两比较采用单因素方差分析。结果:IL-10mRNA及其蛋白在牙周局部牙龈组织均有表达,表达细胞类型有淋巴细胞、浆细胞、巨噬细胞及成纤维细胞等。IL-10在mRNA水平及蛋白水平表达无显著差异(P>0.05)(牙龈炎组P<0.05)。牙周炎组IL-10表达强度显著高于健康对照组和牙龈炎组(P<0.01),牙周炎组IL-10mRNA表达强度显著高于健康对照组(P<0.01),但与牙龈炎组比较差异无显著性(P>0.05)。结论:IL-10在牙周组织中存在局部分泌机制。PURPOSE:To investigate the cell type which express IL-10 mRNA and the protein in gingival tissues of patients with chronic periodontitis. METHODS:12 patients with chronic periodontitis who were undergoing flap operation were randomly chosen as periodontitis group;10 patients undergoing gingivectomy were chosen as gingivitis group;6 patients undergoing extraction of impacted teeth were chosen as control group. Assays of in situ hybridization and immunohistochemistry were used to observe the expression of IL-10 mRNA and the protein in gingival tissues of each group respectively . Wilcoxon test was used to compare the expression of IL-10 of each group at two levels; differences between the two groups were analyzed by one-way ANOVA test. RESULTS: IL-10 mRNA and the protein were all expressed in gingival tissues of periodontium,they were found in lymphocytes, fibroblasts and macrophages. There were no significant differences between two levels of IL-10 expression of periodontitis group and control group(P>0.05)(gingivitis group,P<0.05). The IL-10 expression of periodontitis group was significantly higher than gingivitis group and control group (P<0.01); The IL-10 mRNA expression of periodontitis group was significantly higher than control group(P<0.01),but there was no significant difference between the IL-10 mRNA expression of periodontitis group and gingivitis group (P >0.05). CONCLUSION: There is a local secretion mechanism of IL-10 in the periodontal tissues.

关 键 词：白细胞介素-10 慢性牙周炎 牙龈组织 原位杂交 免疫组化 

分 类 号：R781.42[医药卫生—口腔医学]