绵羊左心衰竭模型的建立  

Establishment of Left Ventricular Failure Model in Sheep

作　　者：潘黎明[1] 吴信[1] 周维新[1] 吕凤英[1] 唐跃[1] 孟亮[1] 周燕文[1] 

机构地区：[1]中国医学科学院中国协和医科大学心血管病研究所阜外心血管病医院外科,北京100037

出　　处：《中国胸心血管外科临床杂志》2005年第3期177-180,共4页Chinese Journal of Clinical Thoracic and Cardiovascular Surgery

基　　金：国家自然科学基金资助项目(30371409)~~

摘　　要：目的通过选择性结扎绵羊冠状动脉对角支,以获得稳定的心力衰竭动物模型。方法对4只绵羊从心尖至心底大约40%处结扎冠状动脉对角支,在结扎前、结扎后30min和7d观察血流动力学指标的变化,作彩色超声心动图检查,记录心电图,评价心功能;然后处死绵羊,作心肌病理检查。结果4只绵羊均存活。结扎后30min和7d主动脉收缩压、心排血量较结扎前降低,肺动脉收缩压、肺毛细血管楔压和中心静脉压较结扎前均升高;超声心动图检查显示左心室舒张期末内径、左心室收缩期末内径较结扎前增大,左心室射血分数、左心室缩短分数较结扎前降低(P<0.05)。结论通过选择性结扎绵羊冠状动脉对角支可以获得稳定、可靠的左心衰竭动物模型。Objective To report a reliable left heart failure model in sheep using selected ligation of the diagonal branch. Methods Four male sheep were used. After a left anterior thoracotomy in sheep, the diagonal branch of coronary artery was ligated at a point approximately 40% of the distance from the apex to the base of the heart. Hemodynamic and echocardiography measurements were done preligation, 30 minutes and 7 days after the coronary artery of diagonal branch ligation. The electrocardiograms were obtained as needed, and cardiac function was also evaluated. The sheep were killed for postmortem examination of their hearts. Results Four sheep survived the experimental procedures. Comparing with before surgery, systemic arterial blood pressure and cardiac output were decreased, pulmonaryartery systolic pressure, pulmonary capillary wedge pressure and central venous pressure were increased at 30 min and 7 days after selected ligation of the coronary artery of diagonal branch; left ventricular end-diastolic dimension and left ventricular end-systolic dimension were increased; left ventricular ejection fraction and left ventricular fractional shortening were also decreased (P<0.05). Conclusion A reliable ovine model of left ventricular failure using selected ligation of the diagonal branch of the coronary artery can be achieved. This animal model is comparable to the clinical correlation.

关 键 词：左心衰竭 绵羊 超声心动图检查 血流动力学指标 肺毛细血管楔压 左心室射血分数 冠状动脉 肺动脉收缩压 动物模型 中心静脉压 心力衰竭 病理检查 心排血量 舒张期末 收缩期末 缩短分数 结扎后 心电图 心功能 内径 

分 类 号：R-332[医药卫生]