高糖、肿瘤坏死因子促进血管内皮细胞表面整合素（α_vβ_3）表达  

Promoting Effect of Tumor Necrosis Factor and High Glucose on the Expression of Integrin-α_vβ_3 on the Surface of Endothelial Cells

作　　者：栾传荣[1] 隋广智[1] 杜学良[1] 

机构地区：[1]齐齐哈尔医学院组织胚胎学教研室

出　　处：《解剖科学进展》1996年第4期370-376,共7页Progress of Anatomical Sciences

摘　　要：本文采用ＥＬＩＳＡ方法检测培养的人脐静脉内皮细胞（ＨＵＶＥＣ）表面玻璃连接蛋白受体（ＶｉｔｒｏｎｅｃｔｉｎｒｅｃｅｐｔｏｒＶｎＲ即整合素家庭的α＿ｖβ＿３）的表达改变；用（５１）Ｃｒ－标记血小板（（５１）Ｃｒ－ｐｌａｔｅｌｅｔｅ，（５１）Ｃｒ－ｐＬ）检测ＨＵｖＥｃ的粘附功能；Ｆｕｒａ－２／Ａｍ负载ＥＣ，测定ＨＵＶＥＣ胞内游离钙离子浓度（［Ｃａ（２＋）］），观察了高糖、肿瘤坏死因子－α（Ｔｕｍｏｒｎｅｃｒｏｓｉｓｆａｃｔｏｒ－α，ＴＮＦ－α）对ＥＣ粘附功能的影响。结果表明：①高糖（３０ｍｍｏｌ／Ｌ）可明显促进ＥＣ与ＰＬ的粘附（ＣＰＭ值：３６１±２ｌ．９３ＶＳ２ｌ９．６７±１６．２６，ｎ＝６Ｐ＜０．０１），抗β３亚单位单抗（β３ＭｃＡｂ）可部分阻断ＥＣ与ＰＬ粘附。ＴＮＦ－α（１０００μ／ｍ１）也有相同作用（ＣＰＭ值：４ｌ０．７±１７．６ＶＳ２１９．６７±１６．２６１ｎ＝６Ｐ＜０．０１），β３ＭｃＡｂ也部分阻断ＴＮＦ－α诱导的ＥＣ－ＰＬ间的粘附。②不同浓度高糖和ＴＮＦ－α不同时间可影响ＥＣ表面的α＿ｖβ＿３表达，并且在一定范围内有浓度和时间依赖性。③高糖和ＴＮＦ－α可明显增加ＥＣ的［Ｃａ（２＋）］ｉ，上述资料提示?his study was performed on the cultured endothelial cells(EC)to observe the promoting effect oftumor necrosis factor-α(TNF-α) and high glucose (HG) on the expression of integrin-αvβ3 on the surfaceof EC, It was found that TNF-αand HG enhanced obviously the expression of αvβ3, and the adhessivefunction of EC to platelet (PL). With ELIsA, we found that different concentration of TNF-αand HGcould increase the expression of αvβ3 In the same concentration group, the expressiozi of αvβ3 showed aup-tendency with the stimulated time prolonged. In addition, TNF-αand HG can induce the increase of[Ca2+]i remarkably, Our results suggested that TNF-α and HG could strengthen the expression of αvβ3and increase the adhesive function of ECs to Pls。αvβ3was a important mediator in the adhesion of PL-EC。Meanwhile,[Ca2+]i also played a important role in mediating adhesion between ECs and PLs as asecond-messeger。

关 键 词：高糖 肿瘤坏死因子 血管内皮细胞 表面整合素 ΑVΒ3 基因表达 钙离子 

分 类 号：R331.32[医药卫生—人体生理学]