用非放射性标记MYO探针进行DNA指纹分析  

ANALYSIS OF DNA FINGERPRINTS WITH NONRADIOACTIVE LABELLED PROBE MYO

作　　者：邹浪萍[1] 申滨[1] 褚嘉佑[1] 申志明 李长久 龚文明 杨松 

机构地区：[1]云南省玉溪地区公安处,昆明医学院法医学系,中国医学科学院医学生物研究所

出　　处：《法医学杂志》1994年第3期100-103,F003,共5页Journal of Forensic Medicine

基　　金：云南省应用基础研究基金

摘　　要：用非放射性标记ＭＹＯ探针进行人的ＤＮＡ指纹分析，获得了清晰易读的ＤＮＡ指纹图，并用该方法调查了云南省的７８名无关个体，经统计学分析，计算出无关个体的相关几率是３．４×１０－１０，高于３２Ｐ标记的Ｍｙｏ探针。研究结果表明该方法是一种简便、快速、灵敏、可靠、经济的ＤＮＡ指纹图检测技术，可在法医鉴定及其它领域里得到更广泛的应用。Clear and high distinctive patterns of human DNA finger prints were obtained by using nonradioactive labelled probe Myo . The DNA fingerprints of 78 unrelated indiveduals living in Yunnan province of China were detected, and their probability of chance association between random individuals was calculated as 3. 4×10-10. It was higher than that by using radioactive 32 P labelled probe. The result of this study showed that the method by use of nontadioactive labelled probe was simple, fast, sensitive, reliabel and economical. The method can be broadly applied in forensic medicine and other fields.

关 键 词：DNA指纹分析 非放射性标记 MYO 探针 DNA指纹图 经统计学分析 ^32P标记 方法调查 检测技术 法医鉴定 云南省 MYO 个体 

分 类 号：R994.6[医药卫生—毒理学] R392-33[医药卫生—药学]