富含谷氨酰胺二肽的20种结晶氨基酸对短肠综合征大鼠免疫功能的影响  

The effect with 20 amino acid enriched with glutamine dipeptide and branched-chain amino acid on immunity of short bowel syndrome rats

作　　者：王钢乐[1] 宋廷惠[1] 靳继德[2] 尉承泽[1] 张松[3] 徐琪寿[2] 

机构地区：[1]北京军事医学科学院附属医院外一科,100850 [2]北京放射医学研究所 [3]北京空军总医院营养科

出　　处：《中国临床营养杂志》2000年第4期235-238,共4页Chinese Journal of Clinical Nutrition

摘　　要：目的　观察富含谷氨酰胺二肽和支链氨基酸的新型 2 0种结晶氨基酸配制的全肠外营养液对短肠综合征大鼠免疫功能的影响。　方法　 40只Wistar雄性大鼠随机分为谷氨酰胺二肽组、标准组、无氮组及虚拟对照组 ,观察各组动物之间外周血T淋巴细胞转化率、T淋巴细胞亚群 (CD4+ /CD8+ )以及血清肿瘤坏死因子 (TNF)等免疫指标的变化。　结果　谷氨酰胺二肽组与标准组及无氮组相比 ,T淋巴细胞转化率、CD4+ /CD8+及TNF均有不同程度的增高。　结论　富含谷氨酰胺二肽和支链氨基酸的 2 0种结晶氨基酸更有利于手术后创伤应激期免疫功能的恢复 ,增强其生存能力。Objective 40 male Wistars rats were randomly allocated into 4 groups:12 rats in Gln group,12 rats in Std group,8 rats in Nitrogen free group and 8 rats in sham control group.The purpose of this study is to investigate the effects of 20 amino acids (20AA) enriched with glutamine dipeptide and branched chain amino acids on T lymphocyte blastogensis stimulated by ConA,CD4+/CD8+,and the serum TNF of rats receiving small bowel reserction and TPN.The results showed that CD4+/CD8+ of Tcell subgroup ,the serum TNF and T lymphocyte blastogensis stimulated by ConA in Gln TPN group were increased compared with Std TPN group and nitrogen free group, It was concluded that the new 20AA was more beneficial for celluer immunity recovery than 17AA after the major abdomen surgery and serious trauma.It could improved the survival rate of short bowel syndrome (SBS ) rats.

关 键 词：谷氨酰胺二肽 结晶氨基酸 短肠综合征 免疫功能 肿瘤坏死因子(TNF) T淋巴细胞转化率 T淋巴细胞亚群 支链氨基酸 全肠外营养液 WISTAR 雄性大鼠 免疫指标 不同程度 CD8+ CD4+ 生存能力 对照组 外周血 应激期 手术后 标准 

分 类 号：R459[医药卫生—治疗学]