上海地区人体精子黄递酶_Ⅲ型的遗传多态性研究  

INVESTIGATION ON THE GENETIC POLMORPHISM OF DIA_3 IN MAN IN SHANGHAI DISTRICT

作　　者：周月琴[1] 吴文庆[1] 胡三多[1] 张其英[1] 

机构地区：[1]上海医科大学法医学教研室,上海200032

出　　处：《法医学杂志》1992年第4期148-150,192,共4页Journal of Forensic Medicine

摘　　要：Straub提纯一种与NADH或NADPH氧化有关的电子传递酶，具有黄素蛋白特性，命名为黄递酶(Diaphorase，DIA)。Caldwell等首先报导了黄递酶的遗传多态性，可分1—1型、2—1型和2—2型三型，由一对共显性基因控制，作者认为此酶为精于细胞和睾丸组织所特有，称为精子黄递酶(sperm diaphorase，SD)。We investigated the polymorphism of sperm diaphorase Ⅲ with modified mixed starch gel eletrophoresis and PAGIEF.Among the 378 Chinese adult males in Shanghai population, three phenotypes wrer observed and the gene frequencies of DIA_3 wrer calculted as DIA_3~1 0.7275, DIA_3~2 0.2725(by SGE) and BIA_2~1 0.7228,DIA_3~2 0.2744 and DIA_3~v 0.0054(by PAGIEF).The IEF method is applied to phentype DIA_3 in seminal stain,which phenotypes are demostrated for up to 7 weeks at room temperature and for 11 weeks at 4℃. The DIA^3 in all 30 vaginal swabs were not typed by SGE and 1EF.

关 键 词：多态性研究 NADPH 遗传多态性 黄递酶 电子传递 NADH 蛋白特性 基因控制 睾丸组织 于细胞 1型 

分 类 号：R392.12[医药卫生—免疫学] R446.112[医药卫生—基础医学]