家兔角膜上皮细胞DNA含量与死后经过时间关系的研究  

Experimental Studies on the Relationship Between the Postmortem Interval and the Changes of DNA Content in Rabbits' Cornea Epithelia

作　　者：周建斌[1] 杨松涛[2] 熊石龙[3] 赵云[4] 

机构地区：[1]湖南省长沙市第四医院病理科,湖南长沙410006 [2]安徽中医学院医药经济管理学院,安徽合肥230038 [3]中南大学信息物理工程学院生物医学工程研究所,湖南长沙410083 [4]中南大学湘雅二医院血液科,湖南长沙410011

出　　处：《医学临床研究》2005年第6期752-754,共3页Journal of Clinical Research

摘　　要：【目的】探讨家兔死后角膜上皮细胞DNA含量与死后经过时间的关系。【方法】应用细胞图像分析技术,检测家兔死后不同时间点家兔角膜上皮细胞核DNA含量,观察其变化规律。【结果】家兔角膜上皮细胞DNA含量随死亡时间增加而逐渐下降,呈负相关关系(R=-0.773,P=0.003)。【结论】家兔死后角膜细胞DNA含量与死后经过时间有明显的相关性,可用于更可靠、更准确地推断死亡时间。[Objective]To investigate the relationship between postmortem interval (PMI) and the changes of DNA content in the rabbits` cornea epithelia.[Methods]DNA contents and the rule of their variation in cornea epithelia were detected at 12-hour-interval within 72 hours after death (without decay) by histochemical assay and image analysis.[Results]There was a descendent trend of the amount of DNA in cells after different PMI, and the degradation rate of nuclear DNA was negatively correlated with the length of PMI ( R=-0.773, P =0.003).[Conclusion]Measuring the amount of DNA in cornea epithelia of rabbit may offer an objective and accurate method for the determination of PMIs.

关 键 词：上皮 角膜/细胞学 DNA 兔 

分 类 号：R77[医药卫生—眼科]