Riluzole对大鼠早期创伤性脑水肿作用的实验研究  

Experimental Study of Therapeutic Efficacy of Riluzole on Trauamtic Brain Edema at Early Stage in Rats

作　　者：赵贤军[1] 刘运生[1] 裘明德[2] 康笃伦[2] 

机构地区：[1]中南大学湘雅医院神经外科,湖南长沙410008 [2]兰州大学医学院神经病学研究所,甘肃兰州730030

出　　处：《医学临床研究》2005年第6期775-777,共3页Journal of Clinical Research

摘　　要：【目的】探讨电压门控Na+通道拮抗剂riluzole对大鼠早期创伤性脑水肿的作用以及可能的作用机制。【方法】20只Wistar大鼠随机分组,按照Feeney的方法制作颅脑损伤模型,治疗组于损伤后15min、6h、18h、30h分别给予riluzole尾静脉注射。按照设计时段处死动物,取伤灶区脑组织,测定含水量、钠、钙、MDA含量和Na+K+ATPase水平,对其差别进行比较和分析。【结果】假手术组各项指标与正常组比较无明显差异,与正常组比较,损伤组脑组织水、钠、钙、MDA含量升高(P<0.05),Na+K+ATPase活性下降(P<0.05);与损伤组比较,治疗组脑组织含水量下降,于48h差异最为显著(P<0.01),钠、钙、MDA含量降低,而Na+K+ATPase活性改善(P<0.05)。【结论】riluzole可以减轻颅脑损伤后的早期脑水肿,其作用机制可能与抑制电压门控Na+通道的异常过度开放、谷氨酸释放及其兴奋毒性,从而改善能量代谢,减轻自由基反应和钙超载有关。[Objective]To investigate the therapeutic efficacy of sodium channel antagonist riluzole on traumatic brain edema at early stage in rats and probe its possible mechanisms.[Methods]One hundred and twenty Healthy Wistar rats were divided into normal group, sham operation group, damage group and treatment group, after the model were made by Feeney's method(25×30g.cm) on the right parietal cortex. All subjects in treatment group were injected riluzole at the dose of 16mg/kg intravenously 15 minutes, 6 hours, 18 hours and 30 hours after trauma respectively( n =12). The subjects were harvested 6 hours, 12 hours, 24 hours, 48 hours after trauma on schedule, then the activity of sodium potassium triphosphatase, the content of water, sodium, calcium and malondialdehyde in brain tissue around lesion were detected.[Results]In comparison with normal group( n =12), the content of water, sodium, calcium and malondialdehyde in damage group increased remarkably( P <0.05), the activity of sodium potassium triphosphatase decreased significantly( P <0.05). In comparison with damage group, riluzole given intravenously decreased the content of water, sodium, calcium and malondialdehyde( P <0.05), improved the activity of sodium potassium triphosphatase( P <0.05).[Conclusion]Riluzole can attenuate the traumatic brain edema at early stage, the possible mechanism ie as follows: to inhibit the abnormal patency of VDSC, release of glutamate and excitotoxicity, thus to decrease free radicals reaction, the intracellular sodium and calcium overload, to improve the activity of sodium potassium triphosphatase.

关 键 词：脑水肿 脑损伤 大鼠 

分 类 号：R742[医药卫生—神经病学与精神病学]