人血淋巴细胞彗星试验检测南京市水源水的遗传毒性  

Genotoxicity Evaluation of Drinking Water Sources in Nanjing Usingthe Comet Assay with Human Peripheral Blood Lymphocytes

作　　者：厉以强[1] 孙立伟[1] 曲甍甍[1] 陈海刚[1] 李兆利[1] 孔志明[1] 方东[2] 袁洁[2] 

机构地区：[1]污染控制与资源化研究国家重点实验室南京大学环境学院 [2]南京市环境监测中心站,南京210013

出　　处：《南京大学学报（自然科学版）》2005年第3期330-334,共5页Journal of Nanjing University（Natural Science）

基　　金：江苏省科技厅社会发展项目(BS2001039);江苏省环境保护科技发展基金项目(2004012)

摘　　要：　采用人外周血淋巴细胞彗星试验,对N市多个自来水厂水源水中的有机浓集物进行了毒性检测.结果表明,各水样浓集物均能引起不同程度的DNA损伤,与对照组相比均有显著性差异(P<0.05,P<0.01).以每管100mL剂量组进行多重比较发现,不同水厂源水间的遗传毒性存在较大差异,镇级水厂>市级水厂.试验结果与环保部门对各水厂水源水水质检测结果基本一致,证实了彗星试验作为一种简便、快速和灵敏的遗传毒性检测方法可以引入环境监测,能有效检测饮用水源有机浓集物的遗传毒性.<Abstrcat>The toxic potentials of organic pollutants in drinking water source of waterworks of Nanjing were investigated with the comet assay in this paper. The results showed that the DNA damage in human peripheral blood lymphocytes was induced after exposure to the organic extracts from all water samples, and the significant differences of DNA damage were shown between the sample groups and the solvent control groups. The genetic hazard existed. The biggist differences of DNA damage were also shown between different drinking water sources by multiplex comparison at the dose of 100 mL/tube and the genetic hazard of the town's water sources was higher than that of the city. The concordance of the results of the comet assay with the poisonous pollutant monitoring of the water sourceswas well displayed . The results of this study demonstrate that the comet assay can be successfully applied to the genotoxicity monitoring programs of drinking water sources.

关 键 词：饮用水源 遗传毒性 人外周血淋巴细胞 彗星试验 

分 类 号：X172[环境科学与工程—环境科学]