肿瘤浸润淋巴细胞与白介素2联用抑制Lewis肺癌生长和转移的研究  

Study on anti-tumor effect of lewis lung cancer infiltrating-lymphocytes combined with IL-2;suppressing tumor growth and metastasis

作　　者：唐华[1] 陈德基[2] 周洁[3] 刘铭球[2] 张华亭[2] 

机构地区：[1]河南省人民医院病理科,郑州450003 [2]湖北医科大学病理教研室 [3]解放军广州医学高等专科学校

出　　处：《中华实验外科杂志》1998年第2期156-157,共2页Chinese Journal of Experimental Surgery

基　　金：湖北省教委重点项目科研基金

摘　　要：目的研究Lewis肺癌(LLC)肿瘤浸润淋巴细胞(TIL)对荷瘤鼠体内的抗瘤作用。方法从接种于C57BL/6小鼠体内的LLC瘤体中分离提取TIL,经1000u/ml白细胞介素-2 (IL-2)的完全培养基中培养后,与LLC细胞以10∶1接种于C57BL/6小鼠腋下,每日1000u IL-2瘤内注射,于接种后第18天处死小鼠。结果,经TIL和IL-2处理的小鼠肿瘤重量明显低于其它各组(P<0.001),且肺转移发生率也较其它各组低(P<0.001)。结论肿瘤浸润淋巴细胞与IL-2联用可明显抑制LLC的生长和转移。Objective To study the anti-tumor effect of TII, plus IL -2 in vivo Mehtods We implanted tumor infiltrating lymphocytes (TIL) which were isolated from Lewis lung cancer (1.1.C) implahted in C_57BL/6 mice and cultured with IL-2, and LLC cells into C_57BL/6 mice together, which the ratio of TIL: LIC cells being 10 : 1 1000u IL- 2 was intratumorally injelted every day. On the eighteenth day after the cells having been implanted, the mice were killed, tumors were weighted and lung metastases were observed. Results The tumor weight of exeperemental group I(2. 9911. 75) was significantly lower than that of the other groups, and the rate and number of lung metastasis of experemental group I were also significantly lower than that of the other groups. Conclusion TIL plus 11.-2 can suppress tumor growth and metastasis.

关 键 词：肿瘤浸润淋巴细胞 Lewis肺癌 生长和转移 白介素2 抑制 联用 C57BL/6小鼠 C57BL/6小鼠 IL-2 白细胞介素 抗瘤作用 分离提取 瘤内注射 肿瘤重量 TIL 荷瘤鼠 培养基 C细胞 接种后 发生率 肺转移 体内 

分 类 号：R730.5[医药卫生—肿瘤] R734.2[医药卫生—临床医学]