鞘内注射U0126对神经痛大鼠脊髓背角内原癌基因c-fos表达的影响  

Effects of intrathecal injection with U0126 on behavior and c-fos expression in spinal cord in chronic constrictive injury rats

作　　者：宋雪松[1] 李国华[1] 佟丹梅[2] 曹君利[3] 杜宝东[4] 

机构地区：[1]吉林大学第一医院麻醉科,吉林长春130021 [2]吉林大学第四医院麻醉科,吉林长春130011 [3]江苏省麻醉医学研究所江苏省麻醉学重点实验室,江苏徐州221002 [4]吉林大学第一医院耳鼻咽喉-头颈外科,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第3期346-348,364,共4页Journal of Jilin University：Medicine Edition

基　　金：国家自然科学基金青年基金资助课题 (30 2 0 0 2 6 7) ;江苏省教育厅重点实验室开放课题 (KSJ0 30 81)

摘　　要：目的:观察鞘内注射U 0 12 6对神经病理性疼痛大鼠痛行为及脊髓背角内原癌基因c- fos表达的影响。方法:在大鼠慢性压迫性损伤(CCI)模型中采用von Frey纤维丝和热痛刺激仪测定大鼠机械性缩爪潜伏期和热痛缩爪潜伏期,应用免疫组织化学方法检测大鼠脊髓背角内原癌基因c- fos表达的变化。结果:CCI大鼠同侧脊髓背角浅层内Fos阳性神经元明显增多,鞘内应用U 0 12 6能够明显减少背角神经元中Fos表达,同时伴有大鼠痛行为的改善。结论:细胞外调节激酶信号通路在脊髓背角浅层的激活参与神经病理性痛的形成与维持。Objective To investigate the effects of intrathecal injection with U0126 on the behavior of chronic constrictive injury (CCI) rats and the c-fos expression in spinal cord dorsal horn. Methods Thermal and mechanical nociceptive thresholds were measured before CCI and then on odd-numbered days, up to and including 15th day, to assess and compare the anti-nociceptive effects of adminstration with MEK inhibitor U0126. The time course of expression of c-fos were assessed by immunohistochemical analysis.Results Intrathecal adminstration with U0126 could significantly attenuate the activation of c-fos induced by CCI and simultaneously ameliorate mechanical and thermal hyperalgesia induced by CCI. Conclusion This study shows that the activation of extracellular signal-regulate kinase(ERK) contributes to the CCI-induced neuropathic pain.

关 键 词：有丝分裂素激活蛋白酶类 神经痛 痛觉过敏 脊髓 原癌基因蛋白质c—fos 

分 类 号：R-332[医药卫生] R741