血管内皮细胞生长因子对Copenhagen大鼠乳腺癌发生的影响  

Effects of vascular endothelial growth factor on mammary carcinogenesis in Copenhagen rats

作　　者：林哲[1] 吴琼[2] 曲珊珊[2] 全成实[2] 李玉林[2] 

机构地区：[1]吉林大学第二医院普通外科,吉林长春130041 [2]吉林大学基础医学院病理生物学教育部重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第3期372-374,397,共4页Journal of Jilin University：Medicine Edition

基　　金：教育部留学回国科研启动基金 (2 0 0 375 4 )

摘　　要：目的:探讨外源性血管内皮细胞生长因子(VEGF)对致癌剂N- nitroso- N- methylurea(NMU)诱导Copenhagen (COP)大鼠乳腺癌发生的影响。方法:NMU诱导的雌性COP大鼠持续给VEGF后,观察其乳腺癌的发生情况,同时采用RT- PCR及组织病理学等方法,检测大鼠乳腺上皮细胞中VEGF m RNA表达及乳腺组织中MAST细胞的浸润状态。结果:1COP大鼠乳腺癌的发生率在VEGF处理组与未处理组之间差异无显著性(P>0 .0 5 )。2正常青春期COP大鼠乳腺上皮细胞VEGF m RNA的表达与阳性对照组Bufllo(BU F)大鼠相比差异无显著性,但2 30 d后前者的表达明显低于后者(P<0 .0 5 )。3由NMU诱导的COP大鼠乳腺癌上皮细胞中无VEGF的表达,而BU F大鼠有VEGF的阳性表达,两者差异有显著性(P<0 .0 5 )。4 NMU处理的COP大鼠乳腺组织的增大淋巴结中有大量MAST细胞聚集,BU F组无MAST细胞的聚集。结论:外源性VEGF不足以增加COP大鼠乳腺癌的发生率,但内源性VEGF表达减低及免疫反应的增加(MAST细胞聚集)在COP大鼠肿瘤抵抗性方面起一定的作用。Objective To study the effects of extraneous vascular endothelial growth factor (VEGF) on mammary carcinogenesis in resistant Copenhagen(COP)rats induced by N-nitroso-N-methylurea(NMU). Methods VEGF were administered in a sustained releasing formula to female COP rats treated with NMU and mammary tumor incidences in VEGF-treated animals and controls were detected. RT-PCR and histopathology were used for analysis of VEGF expression and MAST cells accumulation. Results ①There was no significance difference of mammary tumor incidence between VEGF-treated animals and controls. ②Mammary epithelial cells from pubescent BUF and COP rats expressed substantial levels of VEGF messages, whereas cells prepared from 230-day old COP rats showed negligible levels of VEGF mRNA. ③Mammary epithelial cells from tumors developed in susceptible BUF rats expressed VEGF, whereas VEGF messages were barely detectable in tumors induced in COP rats. ④Enlargement of the intramammary lymph nodes with prominent MAST cells was observed in NMU-treated COP rats, but not in NMU-treated BUF rats. Conclusion These results suggest that down-regulation of VEGF expression is insufficient for resistance to mammary carcinogenesis, and negative or low expression of endogenic VEGF and enhanced immune response with MAST cells accumulation may also play a role in conferring resistance in the COP rats.

关 键 词：内皮 血管 内皮生长因子 乳腺肿瘤 Copenhagen大鼠 

分 类 号：R-332[医药卫生] R737.9