MMP-9及其mRNA在胃癌组织中的表达及与Ⅳ型胶原的关系  

Expressions of MMP-9 and MMP-9 mRNA in gastric carcinoma and their correlations with type Ⅳ collagen

作　　者：刘湛[1] 王璐[2] 李玉林[2] 张丽红[2] 杨柯[1] 赵雪俭[1] 

机构地区：[1]吉林大学基础医学院病理生理学教研室,吉林长春130021 [2]吉林大学基础医学院病理生物学教育部重点实验室,吉林长春130021

出　　处：《吉林大学学报（医学版）》2005年第3期375-377,共3页Journal of Jilin University：Medicine Edition

基　　金：教育部博士学科点专项科研基金资助课题 (2 0 0 10 1830 0 1)

摘　　要：目的:观察基质金属蛋白酶- 9(MMP- 9)及其m RNA在胃癌中的表达特点,探讨其与 型胶原的破坏程度之间的关系。方法:74例胃癌标本,经免疫组织化学染色、原位杂交及形态定量分析方法,对同一肿瘤组织进行MMP- 9、MMP- 9m RNA、 型胶原的检测,并结合临床病理参数进行综合分析。结果:MMP- 9及其m RNA的表达均与癌组织的浸润深度、分化程度、淋巴结转移有关;随着 型胶原连续性的降低,MMP- 9阳性病例数不断增高,但各组间差异尚无统计学意义。结论:胃癌细胞自身具有产生与分泌MMP- 9的能力,其对肿瘤的浸润、转移具有促进作用;MMP- 9的产生、分泌及发挥降解功效是在机体内环境的严格监视控制之下进行的。Objective To investigate the expressions of MMP-9 and MMP-9 mRNA in gastric carcinoma and their correlations with the degradation of type Ⅳ collagen. Methods The expressions of MMP-9,MMP-9 mRNA and type Ⅳ were observed by immunohistochemistry,in situ hybridization and morphometric analysis in 74 cases of gastric carcinoma.The relationships between the expressions and the clinical parameters were analysed. Results The expressions of MMP-9 and its mRNA were closely related to the invasion, differentiation and lymph nodes status of the gastric carcinoma.With the degradation of type Ⅳ collagen,the MMP-9 positive cases were increased.However,the difference among the groups had no statistical significance. Conclusion Gastric carcinoma cells have the ability to produce and secret MMP-9 which can promote the invasion and metastasis of the neoplasm. The production,secretion and function of MMP-9 were extremely controlled by the regulation of internal environment.

关 键 词：胃肿瘤 明胶酶B 胶原Ⅳ型 

分 类 号：Q556.9[生物学—生物化学] R735.2[医药卫生—肿瘤]