老年人血小板内钙的变化  

Change of Intracellular Calcium in Platelets from Aged Persons

作　　者：曹云鹏[1] 李智[2] 丛华[2] 聂华[2] 魏敏杰[2] 

机构地区：[1]中国医科大学附属第一医院神经内科,辽宁沈阳110001 [2]中国医科大学基础医学院药理学系

出　　处：《中国血液流变学杂志》2005年第1期62-63,共2页Chinese Journal of Hemorheology

摘　　要：目的　观察老年人血小板内钙浓度([Ca2 + ]i)的变化。方法　以Fura/AM为荧光指示剂,采用荧光分光光度计测定分离的老年及成年人血小板内[Ca2 + ]i。结果　在细胞外钙为1mmol·L-1的情况下,静息状态老年人血小板[Ca2 + ]i及对高钾除极化的反应均明显低于青年对照组(P <0 .0 1) ,高钾使血小板内[Ca2 + ]i增加的绝对值在老年人与青年人间无明显差异(P>0 .0 5 )。结论　老年人血小板[Ca2 + ]i降低,但在相同情况下老年人血小板胞内钙超载更加明显,从而较青年人更易发生细胞的损害。Objective To observe the change of intraplateletic free calcium concentration (i) of aging persons.Method Fluorescence spectrophotometer with fura-2 as a calcium fluorant was used to measure i of dissociated platelets from aging person and adults.Result In the presence of extracellular Ca^(2+) 1mmol·L^(-1) resting i and the response of rise in i to KCl in platelets from aged persons were all significantly reduced than those from young persons,but it significantly enhanced the increase of i induced by different concentration of KCl in both group,and the enhancement was not significantly different between the aging and the young(P>0.05).Conclusion Resting platelet i in aging persons is reduced but,plateleti in aging persons overburdened more significantly than that in the young under same circumstances,and then the cells of aging persons more easily get damaged.

关 键 词：老年人 血小板细胞 细胞内钙 

分 类 号：R741.02[医药卫生—神经病学与精神病学]