人成骨细胞胰岛素受体底物的表达  

The Expression of Insulin Receptor Substrate in Human Osteoblast

作　　者：黄秋霞[1] 周后德[1] 廖二元[1] 杜巍[1] 

机构地区：[1]中南大学湘雅二医院代谢内分泌研究所

出　　处：《中国医师杂志》2005年第5期597-599,共3页Journal of Chinese Physician

基　　金：国家自然科学基金资助项目(30400218)

摘　　要：目的观察人成骨肉瘤MG-63细胞和正常人成骨细胞(humanosteoblast-likecellsHOB)胰岛素受体底物(insulinreceptorsubstrateIRS)的表达。方法半定量RT-PCR检测IRSmRNA表达,免疫共沉淀检测IRS蛋白表达。结果人成骨肉瘤MG-63细胞和正常人成骨细胞均有IRS-1、-2、-3、-4mRNA和蛋白质的表达,以IRS-1的表达丰度最高,IRS-4表达丰度最低。其中IRSmRNA和蛋白质在MG-63细胞中的表达丰度较在正常人成骨细胞中的表达丰度稍高。结论人成骨肉瘤MG-63细胞和正常人成骨细胞均可表达IRSmRNA和蛋白质。且IRS不同成员在同一细胞中的表达丰度不同。Objective To determine the expression of insulin receptor substract(IRS) family in human osteosarcoma cell line MG-63 and cultured normal human osteoblast-like cells (HOB). Methods The mRNA and protein expression of IRS family was measured using semi-quantitative RT-PCR and western blot analysis, respectively. Results MG-63 cells and HOB had the mRNA and protein expressions of IRS-1,-2,-3, and -4. Among IRS family, the expressions levels of IRS-1 mRNA and protein were the highest, and those of the IRS-4 mRNA and protein were the lowest in MG-63 cell line and HOB. Conclusion MG-63 and HOB can express the mRNA and protein of IRS family members, and the expressional levels of IRS family members were different.

关 键 词：胰岛素受体底物 人成骨细胞 MG-63细胞 定量RT-PCR检测 人成骨肉瘤 表达丰度 IRS-1 mRNA表达 cells 免疫共沉淀 IRS-4 蛋白质 蛋白表达 正常人 

分 类 号：R738.1[医药卫生—肿瘤]