CIK细胞的诱导及其对肝癌细胞毒作用的体外研究  

The Induction of Cytokine-induced Killer and its Cytotoxic Effect on Hepatic Carcinoma Cells

作　　者：胡礼仪[1] 张有顺[1] 周新[2] 戴宗晴[1] 黄玲[1] 王菊[1] 

机构地区：[1]郧阳医学院附属东风医院肝研所,湖北十堰442008 [2]武汉大学中南医院基因诊断中心

出　　处：《中国医师杂志》2005年第5期604-606,共3页Journal of Chinese Physician

摘　　要：目的体外诱导细胞因子诱导的杀伤细胞(CIK),并研究其生物学活性。方法从外周血和脐血分离单个核细胞(PBMC),经过细胞因子诱导、培养并扩增CIK细胞,以LAK作比较,流式细胞仪检测CIK细胞表面标志CD3、CD56。3H-TdR释放法检测CIK的增殖能力,MTT法检测对肝癌细胞系SMMC-7721、Bel-7402,正常胎肝细胞L-02的杀伤活性。结果CIK细胞第二周进入快速增殖期,到第31d扩增倍数超过60倍,CD3+CD56+细胞扩增倍数达800倍以上;CIK对肝癌细胞的杀伤能力明显优于LAK细胞(65%～81%);对正常胎肝细胞的细胞毒作用(<5%)。结论CIK细胞是一种具有很强杀瘤活性的免疫活性细胞,具有临床应用前景。Objective To prepare cytokine-induced killer(CIK) in vitro, and study the biological activities of CIK. Methods Lymphocytes were isolated from peripheral blood and umbilical blood, and induced by cytokine.The cell surface markers CD_3 and CD_~56 of CIK were analyzed by FACS. The proliferation of CIK was tested using 3H -TdR release method, and the cytotoxic effect of CIK on hepatic carcinoma cells was measured by MTT. Results CIK cells quickly proliferated from the second week, and expanded more than 60-fold at the 31th day after induction. The CD_3+ and CD_~56 + cells expanded more than 800 folds. The cytotoxic effect of CIK on hepatic carcinoma cells was obviously stronger than that of LAK cells, and CIK had not obvious cytotoxic effect on fetal liver cells. Conclusion CIK was a highly efficient cytotoxic cells against tumors, and had clinical application potentials.

关 键 词：CIK细胞 细胞毒作用 体外研究 细胞因子诱导的杀伤细胞 ^3H-TdR释放法 SMMC-7721 Bel-7402 流式细胞仪检测 细胞表面标志 CD56^+ 免疫活性细胞 临床应用前景 胎肝细胞 生物学活性 单个核细胞 肝癌细胞系 CD3^+ 体外诱导 

分 类 号：R735.7[医药卫生—肿瘤]