STI571对急性非淋巴细胞白血病细胞分化及CD_(117)分化抗原影响的研究  

The Effect of STI571 on the CD_(117) Expression and Differentiation of Mononuclear Cells in Patients with Acute Non-lymphocytic Leukemia

作　　者：澹台林芳[1] 冯茹[2] 

机构地区：[1]兰州军区总医院血液科,甘肃兰州730050 [2]第一军医大学附属南方医院血液科

出　　处：《中国医师杂志》2005年第5期615-616,共2页Journal of Chinese Physician

摘　　要：目的探讨STI571对急性非淋巴(ANLL)白血病细胞CD117及原代细胞分化作用的影响。方法不同浓度STI571培养前后采用流式细胞仪测定CD117的表达,涂片吉姆萨、过氧化物酶染色观察细胞的分化情况。结果不同药物浓度组的粒、单核细胞均有不同程度的分化,随着药物浓度的增加,原早幼细胞逐渐减少,中晚幼和成熟细胞数增加,并且过氧化物酶染色阳性改变,有诱导向同系终末分化的作用。CD117的表达于STI571作用后下降,培养后各药物浓度组与培养前之间有显著性差异(P<0.05),而空白对照组与培养前差异不显著(P>0.05);培养后各浓度组与对照之间存在显著性差异(P<0.05),且0.1μmol·L-1与10μmol·L-1的表达存在显著性差异(P<0.05)。结论STI571对急性非淋巴细胞白血病原代细胞有诱导向同系终末分化的作用,对c-kit酪氨酸激酶有明显的抑制作用,且与药物浓度相关。Objective To explore the effect of STI571 on the CD_~117 expression and differentiation of bone marrow mononuclear cells in patients with acute non-lymphocytic leukemia(ANLL). Methods CD_~117 expression before and after STI571 treatment in vitro was assayed by FACS, and the cell differentiation was observed by Giemsa and peroxidase staining. Results After different concentrations of STI571 treatment in vitro, there was different degrees of differentiation in granulocytes and monocytes from patients with ANLL. With the increase of STI571 concentration, the number of progranulocyte and promyelocyte gradually decreased, the number of myelocyte, metamyelocyte and monocyte increased, and the positive degree of peroxidase staining increased. CD_~117 expression significantly decreased after STI571 treatment compared with before it. Conclusion STI571 could induce leukemia cells of ANLL into terminal differentiation by inhibiting c-kit tyrosine kinase.

关 键 词：急性非淋巴细胞白血病 STI571 CD117 影响的研究 分化抗原 细胞分化 显著性差异 过氧化物酶染色 药物浓度 ST1571 原代细胞 终末分化 白血病细胞 流式细胞仪 酪氨酸激酶 c-kit 分化作用 不同浓度 染色观察 不同程度 单核细胞 

分 类 号：R733.71[医药卫生—肿瘤] R733.7[医药卫生—临床医学]