慢性应激对大鼠结肠粘蛋白muc2表达的影响  

The Effect of Chronic Stress on muc2 Protein Expression in Colonic Mucosa of Rats

作　　者：仝巧云[1] 罗和生[2] 朱宗耀[1] 周纳新[1] 盛崇明[1] 

机构地区：[1]三峡大学第一临床医学院湖北省宜昌市中心人民医院消化科,湖北宜昌443000 [2]武汉大学人民医院消化科

出　　处：《中国医师杂志》2005年第5期629-631,共3页Journal of Chinese Physician

摘　　要：目的了解慢性应激对大鼠结肠杯状细胞粘液及粘蛋白含量的影响。方法健康成年Wistar大鼠24只,随机分为实验组和对照组,对照组大鼠自由活动,实验组每天置于束缚笼内限制活动2h,持续14d后处死,粘液组化AB/PAS法观察结肠黏膜含粘液杯状细胞的数目、免疫组化方法测定粘蛋白muc2的蛋白表达情况。结果重复应激引起大鼠结肠含粘液杯状细胞数目减少(33.00±6.31vs47.42±8.06)(t=4.878,P<0.01),杯状细胞内粘蛋白muc2蛋白表达减少(0.22±0.03vs0.26±0.02)(t=4.537,P<0.01)。结论慢性应激影响肠黏膜粘液分泌细胞的功能,损伤肠粘液保护屏障。Objective To investigate the effect of chronic stress on the mucin secretion and muc2 protein content in goblet cells of rat colonic mucosa. Methods 24 healthy Wistar rats were randomly divided into 2 groups. Control group moved freely, experimental group was forced to undergo 2-hour-long immobilization stress per day for 14 days. The number of goblet cells containing mucus was determined by AB/PAS histochemical staining. The expression of muc2 protein was detected by immuohistochemical staining. Results Chronic stress decreased the number of goblet cells containing mucus, and down-regulated the expression of muc2 protein in goblet cells in rat colonic mucosa. Conclusion Chronic stress damaged the colonic epithelial barrier and functions of colonic goblet cells.

关 键 词：慢性应激 MUC2 粘蛋白 成年WISTAR大鼠 免疫组化方法 粘液分泌细胞 杯状细胞 蛋白含量 限制活动 结肠黏膜 粘液组化 表达情况 细胞数目 重复应激 表达减少 对照组 实验组 PAS 2蛋白 细胞内 肠粘液 vs 

分 类 号：R574.6[医药卫生—消化系统]