韦-伯综合征相关印记基因在人类卵母细胞及植入前胚胎的正常表达(英文)  

Expression of imprinted genes related to Beckwith-Wiedemann syndrome in human oocytes and preimplantation embryos

作　　者：沈文洁[1] 邢福祺[1] 孔令红[1] 陈士岭[1] 李红[1] 

机构地区：[1]南方医院生殖中心,广州510515

出　　处：《中华医学遗传学杂志》2005年第3期265-267,共3页Chinese Journal of Medical Genetics

基　　金：广东省科技计划项目基金(2003c34212)~~

摘　　要：目的检测韦伯综合征(BeckwithWiedemannsyndrome,BWS)相关印记基因在人类卵母细胞和植入前胚胎的正常表达,探讨辅助生殖技术(assistedreproductivetechnology,ART)和BWS的关系。方法应用嵌套式逆转录聚合酶链反应技术检测印记基因P57KIP2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。结果卵母细胞和各期植入前胚胎、囊胚泡中均存在P57KIP2表达。LIT1自8细胞胚胎开始表达,持续至囊胚泡期。TSSC3于卵母细胞及各期植入前胚胎中均未表达。结论BWS相关印迹基因LIT1、P57KIP2表达于植入前胚胎,ART技术的体外干预可能影响其印迹基因的正常表达。Objective To investigate the expression of imprinted genes related to Beckwith-Wiedemann syndrome(BWS)in human oocytes and preimplantation embryos for understanding the relationship between assisted reproductive technology (ART) and BWS. Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2,LIT1,TSSC3 in human oocytes and preimplantation embryos. Results Transcripts of P57KIP2 were detected in human oocytes and at all stages of preimplantation embryos. LIT1 was expressed only in stages of 8-cell and blastocyst. Transcripts of TSSC3 could not be detected in human oocytes and preimplantation embryos. Conclusion Transcripts of P57KIP2 and LIT1, imprinted genes related to BWS, were detected in human preimplantation development; ART might affect the epigenetics of imprinted genes in early embryogenesis.

关 键 词：韦-伯综合征 印记基因 人类卵母细胞 植入前胚胎 基因表达 

分 类 号：R321[医药卫生—人体解剖和组织胚胎学]