湖南地区鼻咽癌MHC-Ⅰ类链相关基因A第5外显子微卫星多态性研究  

Polymorphism of short tandem repeat of exon 5 of MHC class-I chain related gene A and association with nasopharyngeal carcinoma in a southern Chinese population

作　　者：田伟[1] 罗奇志[1] 李立新[1] 金和坤[2] 王帆[1] 郭实士[1] 曹亚[3] 

机构地区：[1]中南大学湘雅医学院免疫学教研室,长沙410078 [2]湖南省肿瘤医院放疗科 [3]中南大学湘雅医学院肿瘤研究所

出　　处：《中华医学遗传学杂志》2005年第3期309-312,共4页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金(30300311);湖南省杰出青年科学基金(04JJ1007);中南大学留学回国人员科研基金~~

摘　　要：目的研究MHCⅠ类链相关基因A(MHCclassⅠchainrelatedgeneA,MICA)第5外显子微卫星多态性与湖南地区鼻咽癌之间的相关性。方法应用荧光聚合酶链反应基因扫描技术和聚合酶链反应序列特异性引物技术分析127例湖南地区鼻咽癌患者和112名正常人群MICA基因第5外显子微卫星等位基因及MICA基因缺失(MICADel)频率。结果MICAA9表型频率在患者组(45/127)高于对照组(20/112),相对风险值(relativerisk)为2.524(P=0.001,Pc=0.006);MICAA5.1表型频率在患者组(51/127)低于对照组(69/112),相对风险值为0.418(P=0.0004,Pc=0.0026)。进一步分析发现,MICAA9在男性患者组的表型频率(35/101)高于男性对照组(11/78),相对风险值为3.23(P=0.00095,Pc=0.006);MICAA5.1在男性患者组的表型频率(39/101)低于男性对照组(49/78),相对风险值为0.372(P=0.0007,Pc=0.004);各MICASTR等位基因频率在女性患者组与女性对照组之间的差异无统计学意义(Pc>0.05)。结论湖南地区MICASTR等位基因多态性与鼻咽癌相关,MICAA9是该人群男性个体的一个鼻咽癌遗传易感标记。Objective To explore the association between the short tandem repeat polymorphism of exon 5 of MICA gene(MICA-STR) and nasop hary ngeal carcinoma (NPC)in a southern Chinese population.Methods O ne hundred and twenty-seven consecutive NPC patients and 112 randomly selected normal controls residing in southern China mainland were analyzed for MICA-STR allelic variation and MICA gene deletion by fluorescent polymerase chain reacti on-genescanning and polymerase chain reaction-sequence specific priming. Results MICA*A9 was observed at significantly higher frequency in th e NPC patient group than in the control group (relative risk=2.524,P=0.001, Pc=0.006); whereas MICA*A5.1 was present at significantly lower frequency in the NPC patient group than in the control group(RR=0.418,P=0.0004, P c=0.002 6). Further analysis revealed that MICA*A9 was over-represented in male NPC pat i ents, compared with male controls(RR=3.23,P=0.00095,Pc=0.006); wher eas MICA*A5.1 was present at significantly lower frequency in male NPC patients, compared with male controls(RR=0.372,P=0.0007,Pc=0.004).None of t he MICA-STR variants showed statistically significant frequency difference bet ween female NPC patients and female controls(Pc>0.05).Conclusion MICA-STR polymorphism is associated with NPC, and MICA*A9 is a genetic susce p tibility marker of male individuals for NPC in a southern Chinese population.

关 键 词：湖南 鼻咽癌 MHC-Ⅰ类链相关基因A 第5外显子 微卫星多态性 遗传因素 

分 类 号：R739.63[医药卫生—肿瘤] R394[医药卫生—临床医学]