体外联合应用NS-398与5-FU抑制肝癌HepG2细胞生长的研究  

Inhibitory efficacy of NS-398 combined with 5-FU in vitro on hepatocarcinoma cell growth

作　　者：张岭漪[1] 张有成[1] 傅生军[1] 王祥[1] 马力[1] 

机构地区：[1]兰州大学第二医院肝病科,兰州730030

出　　处：《肿瘤》2005年第3期236-238,共3页Tumor

基　　金：甘肃省教育厅科研资助项目(编号:2003-033-06)

摘　　要：目的研究体外联合应用选择性COX-2抑制剂NS-398与5-氟尿嘧啶(5-FU)对肝癌细胞生长的抑制作用。方法应用MTT法观察NS-398、5-FU及NS-398+5-FU对人肝癌细胞株HepG2的生长抑制作用;通过荧光显微镜、透射电镜、流式细胞仪研究NS-398、5-FU及NS-398+5-FU对HepG2的凋亡诱导作用。结果NS-398和5-FU对肝癌细胞HepG2的生长有显著抑制作用,NS-398和5-FU联合应用时抑制效应较单一应用更为明显。透射电镜、荧光显微镜观察和流式细胞仪检测显示,HepG2细胞经NS-398、5-FU及NS-398+5-FU处理后出现典型的细胞凋亡形态变化和Sub-G1峰。结论联合应用选择性COX-2抑制剂NS-398有增强5-FU抑制肝癌细胞生长的作用。Objective To study the efficacy of NS-398 combined with 5-FU in vitro on hepatocarcinoma cell growth. Methods HepG2 cells were treated by NS-398, 5-FU and NS-398 combined with 5-FU for 72 hours, respectively. MTT cell proliferation assay was used to observed the inhibition of HepG2 after treatment. The apoptosis of HepG2 cells was displayed by fluorescent microscopy, transmission electron microscopy and flow cytometric analysis. Results NS-398 and 5-FU could significantly inhibit HepG2 cell proliferation. Compared with NS-398 or 5-FU treatment alone, the combination of these two agents could significantly enhance the inhibition efficacy on HepG2 cells. The treatment of NS-398, 5-FU and the combination of two agents can obviously induce some apoptotic morphologic changes of HepG2 cells. Cellular nucleus shrinking, nucleus fragments, uniform chromatin agglutinating and chromatin fragmentation were seen under transmission electron microscope. The reduction of nucleus size and dense nucleus were viewed under fluorescent microscope except for NS-398 treatment group. Flow cytometric analysis also showed a typical sub-G1 peak in groups 5-FU and NS-398 combined with 5-FU, as well as the sub-Gl peak was higher in NS-398 combined with 5-FU group than in 5-FU group along with the increased number of apoptotic cells whereas the NS-398 group had never showed a sub-G1 peak. Conclusion COX-2 selective inhibitor NS-398 may in vitro enhance the efficacy of 5-FU anti-hepatocarci-noma.

关 键 词：肝肿瘤 环加氧酶抑制药 氟尿嘧啶 HEPG2细胞 

分 类 号：R735.7[医药卫生—肿瘤] R73-361[医药卫生—临床医学]