大豆异黄酮植物雌激素调节大鼠睾丸间质细胞分泌睾酮的机理  

Mechansim of Soybean Isoflavonic Phytoestrogens on Regulation of Testosterone Secretion in Rat Leydig Cell

作　　者：马海田[1] 韩正康[1] 王国杰[1] 邹思湘[1] 

机构地区：[1]南京农业大学农业部动物生理生化重点开放实验室,南京210095

出　　处：《农业生物技术学报》2005年第2期221-225,共5页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金资助项目(No.39970534)资助

摘　　要：通过检测cAMP的浓度、cAMP依赖性的蛋白激酶A(PKA)活性、睾酮的含量及芳香化酶活性的变化,体外研究了大豆异黄酮调节大鼠睾丸间质细胞分泌睾酮的机制。结果显示:大豆异黄酮能够提高cAMP的含量和PKA的活性,同时抑制了芳香化酶的活性,最终导致间质细胞分泌睾酮增加。提示大豆异黄酮植物雌激素促进睾酮的分泌主要是通过激活间质细胞cAMP/PKA信号通路,并且抑制芳香化酶活性,从而降低睾酮向雌二醇的转化而实现的。Effects of soybean isoflavones on the regulation of testosterone secretion in rat Leydig celll in vitro was studied by assaying the concentrations of cAMP, the activities of protein kinase A (PKA) and aromatase. The result showed that soybean isoflavo- nes could promote cAMP accumulation and PKA activity, inhibite aromatase activity and in the result increase testosterone secretion in rat Leydig cell. These results suggested that soybean isoflavons improved testosterone secretion by activating cAMP/PKA pathway and inhibiting the activity of aromatase, thereby reducing the conversion of testosterone to estradiol.

关 键 词：大豆异黄酮 细胞分泌 睾酮 激素调节 睾丸 大鼠 异黄酮植物雌激素 cAMP 机理 芳香化酶 通过检测 蛋白激酶 体外研究 信号通路 间质细胞 PKA 活性 依赖性 雌二醇 含量 抑制 激活 

分 类 号：Q813[生物学—生物工程] TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]