重组毕氏酵母表达rhs-TRAIL发酵条件的优化研究  

Research on Optimization of Fermentation Conditions for Expression of rhsTRAIL in Recombinant P. pastoris

作　　者：胡书良[1] 陈惠音[1] 李茹冰[2] 孔祥平[2] 

机构地区：[1]华南理工大学轻工食品学院,广东广州510641 [2]中国人民解放军458医院肝病研究所分子室,广东广州510602

出　　处：《现代食品科技》2005年第2期12-14,共3页Modern Food Science and Technology

摘　　要：为了提高重组人可溶性肿瘤坏死因子相关的凋亡诱导配体(rhs-TRAIL,114-281)在毕氏酵母工程菌中的表达量,采用1000mL摇瓶培养体系,优化研究了在不同培养基、pH值、表达时间、甲醇浓度等培养条件下的表达情况。经条件优化,最终确定了培养基为完全培养基BMGY/BMMY,甲醇浓度为0.5%,pH为6.0,表达时间为96h。在此优化条件下,目的蛋白表达量可以达到68mg/L。to increase the expression yield of rhs-TRAIL in recombinant p.pastoris. The rhs-TRAIL was expressed in 1000ml shaking flask. By studying the yield of target protein under different conditions: components of medium, PH value, the time of methanol inducing expression and the concentration of methanol, etal. After optimizing the conditions, we conformed the final medium BMGY/BMMY, methanol concentration 0.5%, optimum PH 6.0 and expression time 96 hours. Under the optimum conditions, the expression yield of target protein is 68mg/L.

关 键 词：毕氏酵母 rhs—TRAIL 发酵条件 目的蛋白 诱导表达 

分 类 号：Q819[生物学—生物工程]