As_2O_3碘油栓塞对兔VX_2肝移植瘤生长及血管新生的作用  

The Effect on Tumor’s Growth and Angiogenesis in Rabbit Hepatic Implantation Tumor After Transcatheter Arterial Embolization Using As_2O_3 and Lipiodol

作　　者：吴文娟[1] 邹英华[2] 刘禧[3] 李海涛[1] 刘向东[1] 杨春[4] 崔慧先[4] 

机构地区：[1]河北医科大学第三医院放射科,河北石家庄050051 [2]北京大学第一医院介入血管外科 [3]第四军医大学唐都医院超声科 [4]河北医科大学解剖教研室

出　　处：《实用放射学杂志》2005年第5期449-453,共5页Journal of Practical Radiology

基　　金：河北省卫生厅科研基金资助项目(课题编号:04019)。

摘　　要：目的通过二维超声及免疫组化方法观察肝动脉As2O3碘油栓塞对兔肝移植瘤生长、微血管密度(MVD)及血管内皮生长因子(VEGF)表达的影响。方法40只家兔肝内肿瘤种植后2周,随机分为5组,经肝动脉插管分别给予不同处理,实验设生理盐水灌注组(A组)、As2O3灌注组(B组)、单纯碘化油栓塞组(C组)、阿霉素+碘化油栓塞(D组)及As2O3+碘化油栓塞组(E组),As2O3的用量为2mg/kg。治疗后1周,采用二维超声检测肿瘤大小,计算肿瘤的生长率,病理观察肿瘤的坏死率,免疫组化方法测定瘤区的MVD及VEGF表达强度。结果治疗后1周,各组肿瘤体积分别为(1.441±0.26)cm3、(1.372±0.28)cm3、(0.578±0.16)cm3、(0.523±0.12)cm3和(0.508±0.10)cm3,各栓塞治疗组,肿瘤生长受到明显抑制;单纯碘化油栓塞及阿霉素+碘化油栓塞后,残余肿瘤区的MVD略有升高,两者分别为23.4±4.7和22.3±5.3,与阴性对照A组MVD为21.8±6.3相比,统计学无显著性意义;两组的VEGF表达强度分别为0.163±0.019和0.160±0.016,高于阴性对照A组(Ρ<0.05),A组的VEGF表达强度为0.140±0.02;As2O3+碘油栓塞组残余瘤区的MVD减低,VEGF表达减弱,两者分别为15.1±3.2和0.102±0.02。MVD与VEGF表达强度之间存在正相关。Objective To investigate the effect of transcatheter arterial chemoembolization (TACE) using As_2O_3 and Lipiodol on tumor growth, microvessel density(MVD) and vascular endothelial growth factor (VEGF) expression in hepatic tumor tissue.Methods Forty New Zealand white rabbits were randomly divided into five groups in 8 each group and VX_2 carcinoma was implanted in the left lobes of the liver. Two weeks later, a catheter was inserted into the hepatic artery of rabbit with VX_2 hepatic tumor and infusion was performed via the hepatic artery using physiological saline (group A), As_2O_3(group B), Lipiodol ( group C ) , ADM adding Lipiodol ( group D ) and As_2O_3 adding Lipiodol ( group E ) . The size of tumors was observed by ultrasonic examination, the volume and growth rates of tumors were calculated, necrotic rates of tumors were assessed using pathological slices way . The value of MVD and expression level of VEGF of tumors were examined using immunohistochemistry one week after treatment . The staining intensity of VEGF was evaluated with a computer-assisted image-analyzer . Results The volume of the tumors was (1.441±0.26) cm 3,(1.372±0.28) cm 3,(0.578±0.16) cm 3,(0.523±0.12) cm 3 and (0.508±0.10) cm 3 in groups A, B, C, D and E one week after treatment, respectively. The growth of tumors was suppressed in embolization treatment groups. MVD was slightly higher in groups C and D compared with that in group A, the value of MVD was 23.4±4.7, 22.3±5.3 and 21.8±6.3 in these groups respectively , there was no statistically significant difference between each other(Ρ> 0.05).Expression of VEGF was enhanced after embolization trea- tment (groups C and D) . The staining intensity of VEGF in groups C and D was 0.163±0.019 and 0.160 ±0.016 respectively , and was higher than that in group A ( 0.140±0.02, P<0.05). The value of MVD and expression level of the VEGF were significantly lower in group E(15.1±3.2 and 0.102±0.02, respectively) compared with those in other groups (P<0.05). There was a positive correl

关 键 词：肝肿瘤 家兔 VX2肿瘤 介入治疗 经肝动脉化疗栓塞 

分 类 号：R735.7[医药卫生—肿瘤] R-332[医药卫生—临床医学]