人源性抗角蛋白Fab抗体脂质体的制备  

Preparation of Human Antikeratin Fab Antibody Liposomes

作　　者：夏汝山[1] 周敏[2] 王刚[1] 管海宏[1] 刘玉峰[1] 高天文[1] 张三奇[2] 

机构地区：[1]第四军医大学西京医院皮肤科,陕西西安710032 [2]第四军医大学西京医院药剂科,陕西西安710032

出　　处：《中国皮肤性病学杂志》2005年第5期261-263,共3页The Chinese Journal of Dermatovenereology

基　　金：国家自然科学基金(30371650);第四军医大学西京医院高新技术课题(XJGX03028M20)

摘　　要：目的制备人源性抗角蛋白Feb抗体(HAKFA)脂质体,检测脂质体的体外释放及包裹和游离抗体的活性,探索HAKFA可行的应用途径.方法采用逆相蒸发与钙融合联合法制备HAKFA脂质体,在透射电镜下观察脂质体颗粒的性状及大小,应用紫外分光光度计检测抗体的包封率,ELISA检测包裹前后的抗体活性.结果本方法制备的脂质体呈现圆形、粒径大小较一致,约50～100nm;HAKFA脂质体包封率达到(60.3±12.7)％;HAKFA脂质体呈现缓释特性并且抗体依然保持良好的抗原结合活性.结论逆相蒸发与钙融合法为制备HAKFA脂质体较理想的方法.Objective To prepare the human anti-keratin Fab antibodies(HAKFA) liposomes and to detect the release of liposomes in vitro and to measure the activity of the encapsulated and dissociative antibody; to explore the possible approach of liposomes in application.Methods The HAKFA liposomes were made by combined calcium-induced fusion with reverse phase evaporation methods. The shape and size of the liposomes granule were observed by transmission electron microscope and the encapsulation efficiency of HAKFA liposome was detected by spectrophotometer. The activities of Fab antibody before and after encapsulation were measured by ELISA. Results The HAKFA liposomes were round and the diameter of granules was unanimous,about 50~100 nm. The encapsulation efficiency of HAKFA liposome reached to (60.3±12.7)%.The antibody liposomes showed the speciality of slowly releasing and the antibody had the antigen binding activity.Conclusion Combined calcium induced fusion with reverse phase evaporation method is a feasible method for the preparation of HAKFA liposomes.

关 键 词：人源性抗体 角蛋白 脂质体 包封率 

分 类 号：Q511[生物学—生物化学] Q545