血管内皮生长因子及其受体在肺癌组织中表达的研究  

A study on the expressions of vascular endothelial growth factor and its receptors in lung carcinoma

作　　者：颜浩[1] 徐淑晖[1] 周黎强[1] 章培[2] 黎怀碧[1] 

机构地区：[1]成都市第二人民医院呼吸内科,四川成都610017 [2]成都市第二人民医院病理科,四川成都610017

出　　处：《中国呼吸与危重监护杂志》2005年第3期189-192,共4页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：成都市科委科研基金资助 (19990 2 3 )

摘　　要：目的　探讨血管内皮生长因子(VEGF)及其受体(Flt 1及Flk 1/KDR)与肺癌临床指标及预后的关系。方法　收集6 0例原发性支气管肺癌标本及10例正常肺组织,应用免疫组化方法检测肺癌组织及正常肺组织VEGF、Flt 1及KDR的表达。结果　肺癌组织VEGF、Flt 1及KDR的表达较正常肺组织增强,阳性率分别为6 1 6 %、4 6 6 %及6 0 0 %。VEGF、Flt 1及KDR在肺癌细胞、血管内皮细胞、间质成纤维细胞均呈阳性表达,以癌细胞表达最强。VEGF及KDR表达在不同淋巴结转移及临床分期之间存在显著性差异。肺癌组织VEGF和KDR的表达具有相关性及一致性。CoxRegression风险比例模型分析提示VEGF表达程度为影响肺癌患者预后的指标。结论　VEGF可由肿瘤细胞、血管内皮细胞、间质成纤维细胞等多种细胞产生。VEGF和KDR在肺癌淋巴结转移及临床分期中起重要作用,VEGF可作为肺癌患者判断预后的可靠指标。Objective To explore the association of vascular endothelial growth factor (VEGF) and its receptors with clinical features and their prognostic value in lung carcinoma.Methods Lung tisuue samples were obtained from 60 patients with lung carcinoma received curative surgery and 10 cases without lung carcinoma.Immunohistochemistry on paraffin section was performed with antibodies to VEGF,Flt-1 and KDR to detect the protein expressions and data were compared.Results VEGF,Flt-1 and KDR were expressed in endothelial cells and fibroblasts but most strongly in tumor cells.Positive expression rates of VEGF,Flt-1 and KDR in lung carcinoma were 61.6%,46.6% and 60.0%,respectively which were higher than those in normal lung tissue.There was significant difference in the expression levels of VEGF and KDR in the patients with or without lymphatic metastasis and between different clinical stage.There was positive correlation between the expression levels of VEGF and KDR in lung carcinoma.By COXs proportional hazard model analysis,the expression level of VEGF as well as clinical stage was a predictive index for the progonsis of lung carcinoma.Conclusion VEGF and KDR may contribute to disease advance and lymphatic metastasis of lung carcinoma.

关 键 词：血管内皮生长因子 受体 肺癌组织 基因表达 VEGF 肿瘤 免疫组化 纤维细胞 

分 类 号：R734.2[医药卫生—肿瘤]